Recombinant Human Endocan Protein Summary
Product Specifications
When 1 x 105 cells/well are added to Recombinant Human Endocan/ESM coated plates, adhesion is induced in a dose dependent manner after a 1 hour incubation at 37 °C. The ED50 for this effect is 2‑10 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.Trp20-Arg184, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1810-EC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1810-EC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Endocan/ESM-1
Endocan, also known as endothelial-cell specific molecule-1 (ESM-1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung (1). Endocan expression has also been detected in different epithelia and in adipocytes (2, 3). The expression of endocan is upregulated by TNF alpha, IL-1 beta or lipopolysaccharide and down-regulated by IFN gamma (1). The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues (1). The DS chain is covalently attached to serine 137 (4). Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen-1, LFA-1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA-1-mediated leukocyte activation (5). Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity (3, 6). Genetically engineered cells overexpressing endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo (3, 6). Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported (2, 6).
- Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
- Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
- Wellner, M., et al. (2003) Horm. Metab. Res. 35:217.
- Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
- Bechard, D. et al. (2001) J. Immunol. 167:3099
- Scherpereel, A. et al. (2003) Cancer Res. 63:6084.
Citations for Recombinant Human Endocan Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration
Authors: Y Ghochani, SD Muthukrish, A Sohrabi, R Kawaguchi, MC Condro, S Bastola, F Gao, Y Qin, J Mottahedeh, ML Iruela-Ari, N Rao, DR Laks, LM Liau, GW Mathern, SA Goldman, ST Carmichael, I Nakano, G Coppola, SK Seidlits, HI Kornblum
Cell Reports, 2022-10-18;41(3):111511.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Circulating proteoglycan endocan mediates EGFR-driven progression of non-small cell lung cancer
Authors: YC Yang, KF Pan, WJ Lee, JH Chang, P Tan, CC Gu, WM Chang, SF Yang, M Hsiao, KT Hua, MH Chien
Cancer Res., 2020-06-19;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Murine glomerular transcriptome links endothelial cell-specific molecule-1 deficiency with susceptibility to diabetic nephropathy
Authors: X Zheng, F Soroush, J Long, ET Hall, PK Adishesha, S Bhattachar, MF Kiani, V Bhalla
PLoS ONE, 2017-09-21;12(9):e0185250.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis.
Authors: Shin JW, Huggenberger R, Detmar M
Blood, 2008-07-09;112(6):2318-26.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Endocan is a VEGF-A and PI3K regulated gene with increased expression in human renal cancer.
Authors: Rennel E, Mellberg S, Dimberg A, Petersson L, Botling J, Ameur A, Westholm JO, Komorowski J, Lassalle P, Cross MJ, Gerwins P
Exp. Cell Res., 2007-02-06;313(7):1285-94.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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