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Recombinant Human EphA5 Protein, CF

Catalog #: 3036-A5
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3036-A5-100
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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EphA5 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human EphA5 at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin-A2 Fc Chimera with a linear range of 0.16-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human EphA5 protein
Ser56-Pro573, with a C-terminal 6-His tag
Accession #
AAI43428
N-terminal Sequence
Analysis
Ser56
Predicted Molecular Mass
58.4 kDa
SDS-PAGE
75-85 kDa, reducing conditions

Product Datasheets

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3036-A5

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3036-A5

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA5

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1‑4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The human EphA5 cDNA encodes a 1037 amino acid (aa) precursor including a 24 aa signal sequence, a 549 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 443 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Multiple splice variants of human EphA5 are produced in brain and differ in their extracellular and cytoplasmic sequences (6). Taking into account differences in splice variants between species, comparable regions of the human EphA5 ECD share greater than 96% aa sequence identity with bovine, mouse, and rat EphA5, approximately 40% aa identity with human EphA1 and EphA2, and 54‑61% aa identity with human EphA3, 4, 7, and 8. EphA5 is widely expressed in neurons of the adult human CNS (6), with the exception of few areas such as the substantia nigra (7). In rat, the expression of EphA5 during embryogenesis suggests a role in the development of CNS neurons (8).

References
  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev 15:419.
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
  5. Fox, G.M. et al. (1995) Oncogene 10:897.
  6. Miescher, G.C. et al. (1997) Mol. Brain Res. 46:17.
  7. Olivieri, G. and G.C. Miescher (1999) J. Histochem. Cytochem. 47:855.
  8. Taylor, V. et al. (1994) Neuroscience 63:163.
Entrez Gene IDs
2044 (Human); 13839 (Mouse); 79208 (Rat)
Alternate Names
Bsk; Cek7; EC 2.7.10; EC 2.7.10.1; Ehk1; EHK-1; EHK1Hek7; EK7; EPH homology kinase 1; Eph homology kinase-1; EPH receptor A5; EphA5; EPH-like kinase 7; ephrin type-A receptor 5; Hek7; receptor protein-tyrosine kinase HEK7; Rek7; TYRO4HEK7CEK7; tyrosine-protein kinase receptor EHK-1

Citations for Recombinant Human EphA5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
    Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
    The Journal of Biological Chemistry, 2022-03-10;0(0):101817.
    Species: N/A
    Sample Types: Protein
    Applications: ELISA Capture
  2. Selection of phage-displayed accessible recombinant targeted antibodies (SPARTA): methodology and applications
    Authors: S D'Angelo, FI Staquicini, F Ferrara, DI Staquicini, G Sharma, CA Tarleton, H Nguyen, LA Naranjo, RL Sidman, W Arap, AR Bradbury, R Pasqualini
    JCI Insight, 2018-05-03;3(9):.
    Species: Human
    Sample Types: Phage Antibody Library
    Applications: Bioassay
  3. Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer.
    Authors: Staquicini F, Qian M, Salameh A, Dobroff A, Edwards J, Cimino D, Moeller B, Kelly P, Nunez M, Tang X, Liu D, Lee J, Hong W, Ferrara F, Bradbury A, Lobb R, Edelman M, Sidman R, Wistuba I, Arap W, Pasqualini R
    J Biol Chem, 2015-01-26;290(12):7345-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Immunization
  4. The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus.
    Authors: Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, Konig S, Ensser A, Myoung J, Brockmeyer NH, Sturzl M, Fleckenstein B, Neipel F
    Nat. Med., 2012-06-01;18(6):961-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

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