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Recombinant Human EphB2 Fc Chimera Protein, CF

Catalog #: 5189-B2
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5189-B2-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human EphB2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human EphB2 Fc Chimera is coated at 2 μg/mL, Recombinant Human Ephrin-B2 Fc Chimera (Catalog # 7397-EB) binds with an apparent Kd <0.1 nM.
Source
Mouse myeloma cell line, NS0-derived human EphB2 protein
Human EphB2
(Val19-Leu543)
Accession # P29323		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
P29323
N-terminal Sequence
Analysis
Val19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
84.7 kDa (monomer)
SDS-PAGE
103-108 kDa, reducing conditions

Product Datasheets

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5189-B2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5189-B2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphB2

EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a 125 kDa member of the transmembrane Eph receptor tyrosine kinase family that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverse signaling in the ligand-expressing cells through Ephrin (1, 2). Human EphB2 cDNA encodes a 1055 amino acid (aa) precursor, which includes an 18 aa signal sequence, a 525 aa extracellar domain (ECD), a 21 aa transmembrane segment, and a 491 aa cytoplamic domain. The ECD contains a cysteine-rich region followed by two fibronectin type III domains. The cytoplasmic domain contains the tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (3). Human EphB2 shares 99% aa sequence identity with mouse and rat EphB2 within the ECD region. A short isoform that lacks 70 aa at the C-terminus has also been reported (4). Hippocampal neurons can release vesicles containing full length EphB2, and these are taken up by neighboring glial cells (5). EphB2 is expressed on both sides of the neuronal synapse. It controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity
(6-10). EphB2 interaction with Ephrin-A5 promotes axonal growth cone collapse, while its interaction with Ephrin-B ligands is required for inner ear, renal, urorectal, and vascular development (6, 11-15). Signaling in Ephrin expressing cells through EphB2-Ephrin complex requires proteolytic cleavage of EphB2 that releases its extracellular domain (16). Following the shedding of the extracellular domain of EphB2, the cytoplasmic domain of EphB2 is released from the plasma membrane by the presenilin-dependent gamma -secretase activity to initiate a signaling cascade in the EphB2 expressing cells (16). Aberrant EphB2 expression and activity are implicated in the progression of several cancers (17).

References
  1. Pasquale, E.B. (2008) Cell 133:38.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Fox, G.M. et al. (1995) Oncogene 10:897.
  4. Tang, X.X. et al. (1998) Oncogene, 17:521.
  5. Lauterbach, J. and R. Klein (2006) J. Neurosci. 26:11575.
  6. Cowan C.A. et al. (2000) Neuron 26:417.
  7. Bouvier, D. et al. (2008) J. Neurochem. 106:682.
  8. Cramer, K.S. et al. (2006) Dev. Biol. 295:76.
  9. Katakowski, M. et al. (2005) Neurosci. Lett. 385:204.
  10. Henderson, J.T. et al. (2001) Neuron 32:1041.
  11. Himanen, J.-P. et al. (2004) Nat. Neurosci. 7:501.
  12. Dravis, C. et al. (2007) Hear. Res. 223:93.
  13. Dravis, C. et al. (2004) Dev. Biol. 271:272.
  14. Ogawa, K. et al. (2006) J. Cell Sci. 119:559.
  15. Salvucci, O. et al. (2006) Blood 108:2914.
  16. Litterst, C. et al. (2007) J. Biol. Chem. 282:16155.
  17. Castano, J. et al. (2008) Histol. Histopathol. 23:1011.
Long Name
Eph Receptor B2
Entrez Gene IDs
2048 (Human); 13844 (Mouse)
Alternate Names
CAPB; Cek5; Drt; DRTEphB2; EC 2.7.10; EC 2.7.10.1; EK5; elk-related tyrosine kinase; EPH receptor B2; eph tyrosine kinase 3; EphB2; EPH-like kinase 5; ephrin type-B receptor 2; EPHT3MGC87492; EPTH3; Erk; ERKHek5; Hek5; Nuk; PCBC; protein-tyrosine kinase HEK5; Qek2; Renal carcinoma antigen NY-REN-47; Sek3; Tyro5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5

Citations for Recombinant Human EphB2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The Sequence Basis for Selectivity of Ephrin-B2 Ligand for Eph Receptors and Pathogenic Henipavirus G Glycoproteins
    Authors: Narayanan, KK;Amaya, M;Tsang, N;Yin, R;Jays, A;Broder, CC;Shukla, D;Procko, E;
    bioRxiv : the preprint server for biology
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands
    Authors: D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils
    J. Leukoc. Biol., 2020-04-26;0(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  3. Trivalent CAR T cells overcome interpatient antigenic variability in glioblastoma.
    Authors: Bielamowicz K, Fousek K, Byrd T, Samaha H, Mukherjee M, Aware N, Wu M, Orange J, Sumazin P, Man T, Joseph S, Hegde M, Ahmed N
    Neuro Oncol, 2018-03-27;20(4):506-518.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Bioassay)
  4. EphB4 forward signalling regulates lymphatic valve development.
    Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
    Nat Commun, 2015-04-13;6(0):6625.
    Species: Human
    Sample Types: Antibody
    Applications: Enzyme Assay

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