Recombinant Human EphB3 Fc Chimera Protein, CF Summary
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Leu38-Ala550
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Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5667-B3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: EphB3
EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 526 amino acid (aa) extracellular domain (ECD) of mature human EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, human EphB3 shares 96% aa sequence identity with mouse and rat EphB3. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6-9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10-12). It directs embyronic neuronal axon pathfinding, and its up-regulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). In non-small cell lung cancer, however, it is up-regulated and can promote tumor progression (15). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10-12, 16).
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- Ji, X.-D. et al. (2011) Cancer Res. 71:1156.
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Citations for Recombinant Human EphB3 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Functional and antigenic landscape of the Nipah virus receptor binding protein
Authors: Larsen, BB;McMahon, T;Brown, JT;Wang, Z;Radford, CE;Crowe, JE;Veesler, D;Bloom, JD;
bioRxiv : the preprint server for biology
Species: Chinese Hamster
Sample Types: Transfected Whole Cells
Applications: Immunocytochemistry -
Plxdc family members are novel receptors for the rhesus monkey rhadinovirus (RRV)
Authors: AK Gro beta kopf, S Schlagowsk, T Fricke, A Ensser, RC Desrosiers, AS Hahn
PloS Pathogens, 2021-03-03;17(3):e1008979.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
EphB4 forward signalling regulates lymphatic valve development.
Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
Nat Commun, 2015-04-13;6(0):6625.
Species: Human
Sample Types: Antibody
Applications: Enzyme Assay -
A quantitative and kinetic fusion protein-triggering assay can discern distinct steps in the nipah virus membrane fusion cascade.
Authors: Aguilar HC, Aspericueta V, Robinson LR
J. Virol., 2010-06-02;84(16):8033-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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We used this protein as a standard for developing an ELISA in combination with mAb (cat. MAB5667) and pAb (cat. AF5667). This combination works well detecting Ephb3 in human serum.
