Recombinant Human Ephrin-A2 Fc Chimera Protein, CF

Catalog #: 7856-A2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7856-A2-050	In Stock	50 ug / $432

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Recombinant Human Ephrin-A2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit proliferation of PC‑3 human prostate cancer cells. The ED₅₀ for this effect is 5-25 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human Ephrin-A2 protein

Human Ephrin-A2 (Glu27-Asn188) Accession # O43921	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

O43921

N-terminal Sequence
Analysis

Starts at Glu27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45.1 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Product Datasheets

7856-A2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7856-A2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Ephrin-A2

Ephrin‑A2, also known as ELF‑1, HEK7‑L, LERK‑6, and EPLG6, is an approximately 20 kDa member of the GPI‑linked Ephrin‑A family of proteins that bind and induce the tyrosine autophosphorylation of Eph receptors. In particular, Ephrin‑A2 preferentially interacts with receptors of the EphA family of proteins. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, axon guidance and cancer progression. (1‑3). Human Ephrin‑A2 is synthesized as a 213 amino acid (aa) preproprecursor that contains a 24 aa signal peptide, a 164 aa mature chain, and a 25 aa C‑terminal propeptide that is removed prior to GPI linkage of Ephrin‑A2 to the membrane (4, 5). The mature region is structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands (1, 3), and shares 93% aa sequence identity with mouse and rat Ephrin‑A2. Ephrin‑A2 is expressed in discrete regions of the developing nervous system and limb buds (6‑9). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (9‑11). Ephrin‑A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (10, 12). Its up‑regulation on astrocytes at sites of optic nerve damage may prevent re‑innervation by retinal ganglion cells (13). Ephrin‑A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin‑A2 and inhibition of progenitor cell proliferation (10). In the developing limbs, Ephrin‑A2 regulates cartilage morphogenesis and the projection of motoneuron axons (8, 9, 14).

References

Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
Astin, J.W. et al. (2010) Nat. Cell Biol. 12:1194.
Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
Aasheim, H.-C. et al. (1998) Biochem. Biophys. Res. Commun. 252:378.
Cerretti, D.P. and N. Nelson (1998) Genomics 47:131.
Cheng, H.-J. et al. (1994) Cell 79:157.
Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
Ohta, K. et al. (1997) Mech. Dev. 64:127.
Wada, N. et al. (2003) Dev. Biol. 264:550.
Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
Holmberg, J. et al. (2005) Genes Dev. 19:462.
Nakamoto, M. et al. (1996) Cell 86:755.
Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.

Entrez Gene IDs

1943 (Human); 13637 (Mouse)

Alternate Names

Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; EphrinA2; Ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6