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Recombinant Human Ephrin-A4 Fc Chimera Biotinylated Protein

Catalog #: BT369
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BT369
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Recombinant Human Ephrin-A4 Fc Chimera Biotinylated Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmEphA7/Fc Chimera at 2 µg/mL (100 µL/well) can bind biotinylated rhEphrin-A4/Fc Chimera with a linear range of 0.16-10 ng/mL. Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Ephrin-A4 protein
Human Ephrin-A4
(Leu26 - Gly171)
Accession # P52798		 IEGRMD Human IgG1
(Pro100 - Lys330) 		6-His tag
N-terminus C-terminus
Accession #
P52798
N-terminal Sequence
Analysis
Leu26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43.7 kDa (monomer)
SDS-PAGE
50 kDa, reducing conditions

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BT369

Product Datasheet
COA
SDS

BT369

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution

Reconstitute with sterile PBS at 100 μg/mL.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A4

Ephrin-A4, also known as LERK-4 and EFL-4 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Entrez Gene IDs
1945 (Human); 13639 (Mouse)
Alternate Names
EFL4; EFL-4; EFNA4; EPH-related receptor tyrosine kinase ligand 4; EphrinA4; Ephrin-A4; EPLG4MGC125826; LERK-4; LERK4FLJ57652; ligand of eph-related kinase 4

Citation for Recombinant Human Ephrin-A4 Fc Chimera Biotinylated Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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