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Recombinant Human Erythropoietin R Protein

Catalog #: 307-ER
8 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
307-ER-050/CF

With Carrier

Catalog # Availability Size / Price Qty
307-ER-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Erythropoietin R Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 60-300 ng/mL in the presence of 0.2 U/mL of rhEpo.
Source
Mouse myeloma cell line, NS0-derived human Erythropoietin R protein
Ala25-Pro250
Accession #
P19235
N-terminal Sequence
Analysis
Ala25
Predicted Molecular Mass
25 kDa
SDS-PAGE
32 kDa, reducing conditions

Product Datasheets

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307-ER (with carrier)

Product Datasheet
COA
SDS

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307-ER/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

307-ER

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

307-ER/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin R

Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). The genes for human and mouse Epo R have been cloned and characterized. The full-length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) residues (a 24 aa residue hydrophobic signal sequence, a 226 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 236 aa residue cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected on human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

References
  1. Barber, D.L. and A.D. D’Andrea (1992) Seminars in Hematology 29:293.
  2. Youssoufian, H. et al. (1993) Blood 9:2223.
  3. Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93 - 104.
  4. Baynes, R.D. et al. (1993) Blood 82:2088.
Long Name
Erythropoietin Receptor
Entrez Gene IDs
2057 (Human); 13857 (Mouse)
Alternate Names
EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

Citations for Recombinant Human Erythropoietin R Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Autoantibodies to Erythropoietin Receptor and Clinical Outcomes in Patients With Type 2 Diabetes and CKD: A Post Hoc Analysis of CREDENCE Trial
    Authors: Koshino, A;Neuen, BL;Oshima, M;Toyama, T;Hara, A;Arnott, C;Neal, B;Jardine, M;Badve, SV;Mahaffey, KW;Pollock, C;Hansen, MK;Wada, T;Heerspink, HJL;
    Kidney international reports
    Species: Human
    Sample Types: Serum
    Applications: Bioassay
  2. Association between Anti-Erythropoietin Receptor Antibodies and Cardiac Function in Patients on Hemodialysis: A Multicenter Cross-Sectional Study
    Authors: Y Mochida, A Hara, M Oka, K Maesato, K Ishioka, H Moriya, M Oshima, T Toyama, S Kitajima, Y Iwata, N Sakai, M Shimizu, Y Koshino, T Ohtake, S Hidaka, S Kobayashi, T Wada
    Biomedicines, 2022-08-26;10(9):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA (Standard)
  3. Comparison of Circulating Biomarkers in Predicting Diabetic Kidney Disease Progression With Autoantibodies to Erythropoietin Receptor
    Authors: M Oshima, A Hara, T Toyama, M Jun, C Pollock, M Jardine, S Harrap, N Poulter, ME Cooper, M Woodward, J Chalmers, V Perkovic, MG Wong, T Wada
    Kidney international reports, 2020-11-10;6(2):284-295.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  4. Zinc Supplementation Stimulates Red Blood Cell Formation in Rats
    Authors: YH Chen, HL Feng, SS Jeng
    Int J Mol Sci, 2018-09-18;19(9):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Erythropoietin promoted the proliferation of hepatocellular carcinoma through hypoxia induced translocation of its specific receptor
    Authors: S Miao, SM Wang, X Cheng, YF Li, QS Zhang, G Li, SQ He, XP Chen, P Wu
    Cancer Cell Int., 2017-12-11;17(0):119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Clinical and Pathological Significance of Autoantibodies to Erythropoietin Receptor in Type 2 Diabetic Patients With CKD
    Authors: A Hara, K Furuichi, A Koshino, H Yasuda, TTT Tran, Y Iwata, N Sakai, M Shimizu, S Kaneko, H Nakamura, T Wada
    Kidney Int Rep, 2017-09-08;3(1):133-141.
    Applications: Bioassay
  7. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010-02-16;5(2):e9246.
    Applications: ELISA (Standard)
  8. Erythropoietin administration protects retinal neurons from acute ischemia-reperfusion injury.
    Authors: Junk AK, Mammis A, Savitz SI, Singh M, Roth S, Malhotra S, Rosenbaum PS, Cerami A, Brines M, Rosenbaum DM
    Proc. Natl. Acad. Sci. U.S.A., 2002-07-18;99(16):10659-64.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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