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Recombinant Human F-Spondin/SPON1 Protein

Catalog #: 3135-SP
2 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
3135-SP-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3135-SP-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human F-Spondin/SPON1 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived human F-Spondin/SPON1 protein
Phe29-Cys807, with a C-terminal 6-His tag
Accession #
Q9HCB6
N-terminal Sequence
Analysis
Phe29
Predicted Molecular Mass
88.9 kDa
SDS-PAGE
110-115 kDa, reducing conditions

Product Datasheets

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3135-SP (with carrier)

Product Datasheet
COA
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3135-SP/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3135-SP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3135-SP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: F-Spondin/SPON1

F-Spondin (floor plate and thrombospondin homology), also called Spondin-1, SPON1 or VSGP (vascular smooth muscle growth-promoting factor), is an approximately 110 kDa secreted glycoprotein that is a member of a subgroup of TSR (thrombospondin) molecules that are either membrane-bound or associated with the extracellular matrix (ECM) (1-3). Human F-Spondin is synthesized as an 807 amino acid (aa) precursor with a 779 aa mature region that includes an N-terminal reelin‑like domain, an F-spondin (FS) domain, and six C‑terminal thrombospondin (TSP) type I repeats (1-3). Mature human F-Spondin shares 97%, 97%, 98% and 99% aa sequence identity with mouse, rat, bovine and canine F-Spondin, respectively.  TSP 5 and 6 bind ECM, while TSP 1-4 plus the FS domain may mediate repulsive activity on motor neurons and outgrowth promoting activity on sensory neurons during development or after injury (2-5). Crystal structure indicates that the reelin-like domain binds heparin and may mediate weak dimerization (6). Plasmin cleavage generates a diffusible 95 kDa, 656 aa F-spondin that lacks TSP 5 and 6, while non‑plasmin cleavage between the FS segment and the first TSP repeat generates 60 kDa and 50 kDa fragments (3, 4, 7). F-Spondin shows unusual C‑mannosylation and O-fucosylation within the TSP repeats (3). Mammalian cells expressing F-spondin include floor plate epithelium, ventral motor neurons, Schwann cells, fibroblasts, hippocampal pyramidal cells, endothelial cells, vascular smooth muscle cells and some tumor cells (2-5, 8). F-Spondin can either tether cells to the ECM or interfere with integrin adhesion, thus either blocking or allowing nerve or vascular endothelial cell migration (3, 9). It binds beta -amyloid fibrils and inhibits beta ‑secretase cleavage, thus reducing A beta plaque deposition associated with Alzheimer’s disease (10, 11). F-Spondin is also reported to inhibit differentiation or migration during angiogenesis (affecting endothelial cells) and bone development (affecting osteoclast and chondrocyte precursors) (3, 9, 12, 13).

References
  1. Miyamoto, K. et al. (2001) Arch. Biochem. Biophys. 390:93.
  2. Klar, A. et al. (1992) Cell 69:95.
  3. Feinstein, Y. and A. Klar (2004) Int. J. Biochem. Cell Biol. 36:975.
  4. Burstyn-Cohen, T. et al. (1998) J. Neurosci. 18:8875.
  5. Feinstein, Y. et al. (1999) Development 126:3637.
  6. Tan, K. et al. (2008) J. Mol. Biol. 381:1213.
  7. Tzarfaty-Majar, V. et al. (2001) J. Biol. Chem. 276:28233.
  8. Pyle-Chenault, R.A. et al. (2005) Tumor Biol. 26:245.
  9. Terai, Y. et al. (2001) J. Cell Physiol. 188:394.
  10. Ho, A. and T.C. Sudhof (2004) Proc. Natl. Acad. Sci. USA 101:2548.
  11. Hafez, D.M. et al. (2012) Neuroscience 223:465.
  12. Palmer, G.D. et al. (2010) J. Orthop. Res. 28:1323.
  13. Oka, H. et al. (2011) J. Periodontol. 82:1776.
Entrez Gene IDs
10418 (Human); 233744 (Mouse); 64456 (Rat)
Alternate Names
FSpondin; F-Spondin; KIAA0762VSGP/F-spondin; MGC10724; SPON1; spondin 1, (f-spondin) extracellular matrix protein; spondin 1, extracellular matrix protein; spondin-1; Vascular smooth muscle cell growth-promoting factor; VSGP

Citations for Recombinant Human F-Spondin/SPON1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma
    Authors: J Song, SL Merbs, LJ Sokoll, DW Chan, Z Zhang
    Clin Proteomics, 2019-03-05;16(0):10.
    Species: Human
    Sample Types: Serum
  2. F-spondin plays a critical role in murine neuroblastoma survival by maintaining IL-6 expression.
    Authors: Cheng YC, Liang CM, Chen YP, Tsai IH, Kuo CC, Liang SM
    J. Neurochem., 2009-06-22;110(3):947-55.
    Applications: Western Blot

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