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Recombinant Human Fc alpha/mu R His-tag Protein, CF

Catalog #: 9278-FC Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9278-FC-050
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc alpha/mu R His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Human IgM is immobilized at 2.5 μg/mL, 100 μL/well, the concentration of Recombinant Human Fc alpha /μ R that produces 50% of the optimal binding response is approximately 0.12-0.72 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Fc alpha/mu R protein
Leu17-Arg450, with a C-terminal 6-His tag
Accession #
Q8WWV6
N-terminal Sequence
Analysis
Leu17
Predicted Molecular Mass
47 kDa
SDS-PAGE
84-108 kDa, reducing conditions

Product Datasheets

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9278-FC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9278-FC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc alpha/mu R

Fc alpha/mu Receptor (FCAMR), designated CD351, is an approximately 60 kDa transmembrane protein that serves as a receptor for IgA and IgM immunoglobulins (1). Mature human FCAMR consists of a 434 amino acid (aa) extracellular domain with one Ig-like domain, a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain (2). Within the ECD, human FCAMR shares 51% aa sequence identity with mouse and rat FCAMR. Alternative splicing generates additional isoforms that are truncated following the Ig-like domain, are truncated before the transmembrane segment, or carry a 9 aa deletion and a substitution of the transmembrane segment. FCAMR is expressed on B cells, macrophages, and kidney mesangial cells (2-4). It binds to both IgA and IgM in immune complexes but not to monomeric immunoglobulin (2, 5). FCAMR participates in pathogen clearance as well as foam cell formation by mediating the internalization of IgM-opsonized microbes and oxidized LDL-containing particles (2, 6).

References
  1. Wang, H. et al. (2016) Front. Immunol. 7:99.
  2. Shibuya, A. et al. (2000) Nat. Immunol. 1:441.
  3. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  4. McDonald, K.J. et al. (2002) Biochem. Biophys. Res. Commun. 290:438.
  5. Ghumra, A. et al. (2009) Eur. J. Immunol. 39:1147.
  6. Feng, X. et al. (2010) Atherosclerosis 208:396.
Long Name
Fc alpha/mu Receptor
Entrez Gene IDs
83953 (Human); 64435 (Mouse); 681070 (Rat)
Alternate Names
CD351 antigen; CD351; Fc alpha/mu R; Fc alpha/mu receptor; Fc receptor, IgA, IgM, high affinity; FCA/MR; FCAMR; FKSG87; high affinity immunoglobulin alpha and immunoglobulin mu Fc receptor; immunity related factor; receptor for Fc fragment of IgA and IgM; uR

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