Recombinant Human Fc epsilon RI alpha Fc Chimera Protein, CF New

Catalog #: 11561-FC Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11561-FC-050

Recombinant Human Fc epsilon RI alpha Fc Chimera Protein Binding Activity.

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All Fc epsilon RI alpha Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc epsilon RI alpha Fc Chimera Protein, CF Summary

Product Specifications

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human Fc epsilon RI alpha Fc Chimera (Catalog # 11561-FC) binds to human IgE with an ED₅₀ of less than 10 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Fc epsilon RI alpha protein

Human FcER1 alpha (Val 26-Gln 205) Accession # P12319.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

N-terminal Sequence
Analysis

Val 26

Structure / Form

Disulfide-linked Homodimer

Predicted Molecular Mass

48 kDa

SDS-PAGE

78-86 kDa, under reducing conditions

Product Datasheets

11561-FC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11561-FC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Recombinant Human Fc epsilon RI alpha Fc Chimera (Catalog # 11561-FC) binds to human IgE with an ED₅₀ of less than 10 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Fc epsilon RI alpha Fc Chimera Protein (Catalog # 11561-FC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-86 kDa and 160-170 kDa, respectively.

Reconstitution Calculator

Background: Fc epsilon RI alpha

The alpha subunit of the high affinity IgE receptor (Fc epsilon RI alpha or Fc epsilon RIA) is an IgE‑binding type I transmembrane glycoprotein of the multichain immune recognition (MIRR) family (1, 2). The receptor, Fc epsilon RI, is a tetrameric complex of one alpha, one beta and two gamma subunits ( alpha beta gamma ₂) on mast cells and basophils (1). An alternate trimeric form ( alpha gamma ₂) is expressed on human, but not rodent, mast cells, basophils, eosinophils and professional antigen presenting cells (3). While the gamma subunit is essential for expression of Fc epsilon RI alpha on the cell surface and for cell signaling, the beta subunit, when present, increases the halflife of the Fc epsilon RI complex on the cell surface (3, 4). An isoform of the beta subunit, beta T, blocks processing of the alpha subunit and its cell surface expression (2, 3, 5). Human Fc epsilon RI alpha cDNA encodes 257 amino acids (aa) including a 25 aa signal sequence, a 180 aa extracellular domain containing two Ig‑like domains that bind IgE and an endoplasmic reticulum retention motif, a 21 aa transmembrane domain with a charged amino acid (Asp219) that contributes to intracellular transport, and a 32 aa cytoplasmic sequence (1, 3, 6). Human Fc epsilon RI alpha shares 50-62% aa sequence identity with mouse, rat, equine, ovine, bovine, porcine and canine Fc epsilon RI alpha. Binding of IgE alone increases surface expression of Fc epsilon RI, while crosslinking of IgE/Fc epsilon RI complexes by IgE ligands (allergens) initiates receptor internalization and signaling (2, 4, 5). Mast cell and basophil activation by IgE/Fc epsilon RI crosslinking causes degranulation, releasing histamine, leukotrienes, prostaglandins, and other mediators of immediate‑type and late‑phase allergic reactions. Circulating autoantibodies that crosslink Fc epsilon RI alpha are often found in patients with chronic urticaria (7). Fc epsilon RI on human antigen presenting cells mediates uptake and processing of allergens for presentation by class II MHC (2, 3). Fc epsilon RI expression on human DC and Langerhans cells is up‑regulated during allergic reactions (atopy) and correlates with serum IgE concentration (3).

References

Shimizu, A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1907.
Abramson, J. and I. Pecht (2007) Immunol. Rev. 217:231.
Kraft, S. and J-P. Kinet (2007) Nat. Rev. Immunol. 7:365.
Yamasaki, S. and T. Saito (2008) J. Pharmacol. Sci. 106:336.
Brenzovich, J. et al. (2009) J. Leukoc. Biol. 86:1351.
Cauvi, D.M. et al. (2006) J. Biol. Chem. 281:10448.
Kikuchi, Y. et al. (2001) J. Allergy Clin. Immunol. 107:1056.

Long Name

Fc epsilon Receptor I alpha

Entrez Gene IDs

2205 (Human); 14125 (Mouse); 25047 (Rat)

Alternate Names

Fc epsilon RI alpha; Fc epsilon RI alpha-chain; Fc fragment of IgE receptor Ia; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; FCE1A; Fc-epsilon RI-alpha; FcepsilonRI alpha chain; FcepsilonRIalpha; FCER1A; FcERI; FceRIa; high affinity immunoglobulin epsilon receptor alpha-subunit; high affinity immunoglobulin epsilon receptor subunit alpha; igE Fc receptor subunit alpha; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide