Recombinant Human FCRL2/FcRH2 Protein, CF Summary
Product Specifications
Leu20-Asp395, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2048-FC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: FCRL2/FcRH2
Fc receptor-like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3 - 5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27+ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the immunoglobulin heavy chain variable (IGHV) and less aggressive forms of B-CLL (9, 11).
- Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
- Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
- Miller, I. et al. (2002) Blood 99:2662.
- Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
- Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768.
- Hatzivassiliou, G. et al. (2001) Immunity 14:277.
- Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
- Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
- Huttmann, A. et al. (2006) Leukemia 20:1774.
- Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
- Li, F.J. et al. (2008) Blood 112:179.
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