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Recombinant Human FGF-12 Protein

Catalog #: 2246-FG
3 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
2246-FG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2246-FG-025
Recombinant Human FGF-12 Protein Binding Activity
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Product Details
Citations (3)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF-12 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human FGFR4 Fc Chimera (Catalog # 685-FR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant FGF-12 binds with an ED50 of 3-18 μg/mL.
Source
E. coli-derived human FGF-12 protein
Met1-Thr181
Accession #
NP_004104
Predicted Molecular Mass
20.4 kDa

Product Datasheets

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2246-FG (with carrier)

Product Datasheet
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SDS

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2246-FG/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2246-FG

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2246-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human FGF-12 Protein Binding Activity View Larger

When Recombinant rhFGF R4/Fc Chimera (685-FR) is immobilized at 2 μg/mL (100 μL/well), it binds to Recombinant Human FGF-12 (Catalog # 2246-FG) with an ED50 of 3-18 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-12

Fibroblast growth factor 12 (FGF-12) is a member of the FGF superfamily of molecules which contains at least 22 members. The FGFs are characterized by the presence of a core, 120 amino acid (aa) beta -trefoil structure, and all apparently bind heparin (1, 2). FGF-11, -12, -13, -14, originally termed FGF homologous factors (FHF) -3, -1, -2, -4, respectively, form a subgroup within the FGF family (1, 3). Human FGF-12/FHF-1 is synthesized as a 243 aa protein. It lacks a typical signal sequence and is considered to be a cytoplasmic protein. It does, however, possess an N-terminal bipartite nuclear localization signal (NLS) at aa 11-18 and 28-38 (4-6). The 243 aa protein has at least one alternate splice form that is 181 aa in length. This is termed FGF-12B. Alternate splicing deletes the N-terminal 66 aa in FGF-12 and replaces them with four aa in FGF-12B. This substitution removes the NLS from the short form. Studies on the short form (12B) show that it cannot bind any of the common FGF receptors. This is consistent with its cytoplasmic localization. It can, however, bind to IB2 (islet brain-2), a cellular kinase scaffold protein, and voltage-gated sodium channels, suggesting FGF-12B plays an important role in intracellular signaling and ion exchange (7). Mouse and human FGF-12B differ by only one amino acid.

References
  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Ornitz, D.M. and N. Itoh (2001) Genome Biol. 2:REVIEWS 3005.
  3. Green, P. et al. (1996) BioEssays 18:639.
  4. Smallwood, P. M. et al. (1996) Proc. Natl. Acad. Sci. 93:9850.
  5. Munoz-Sanjuan, I. et al. (2000) J. Biol. Chem 275:2589.
  6. Kok, L.D.S. et al. (1999) Biochem. Biophys. Res. Commun. 255:717.
  7. Olsen, S. K. et al. (2003) J. Biol. Chem. 278:34226.
Long Name
Fibroblast Growth Factor 12
Entrez Gene IDs
2257 (Human)
Alternate Names
FGF12; FGF-12; FGF12B; FHF1; FHF-1; FHF1Fibroblast growth factor homologous factor 1; fibroblast growth factor 12; fibroblast growth factor 12B; fibroblast growth factor FGF-12b; Myocyte-activating factor

Citations for Recombinant Human FGF-12 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The FGF/FGFR system in the microglial neuroinflammation with Borrelia burgdorferi: likely intersectionality with other neurological conditions
    Authors: G Parthasara, MB Pattison, CC Midkiff
    Journal of Neuroinflammation, 2023-01-17;20(1):10.
    Species: Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010-10-01;177(5):2379-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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