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Recombinant Human FGF-17 Protein

Catalog #: 319-FG
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
319-FG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
319-FG-025
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Recombinant Human FGF-17 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 100‑500 ng/mL, in the presence of 1 µg/mL heparin, in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002) and 15‑60 ng/mL, in the presence of 1 µg/mL heparin, when measured by 3H-thymidine incorporation.
Source
E. coli-derived human FGF-17 protein
Thr23-Thr216, with and without an N-terminal Met
Accession #
O60258
N-terminal Sequence
Analysis
Met & Thr23
Predicted Molecular Mass
22.6 kDa
SDS-PAGE
21.5 & 22.6 kDa, reducing conditions

Product Datasheets

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319-FG (with carrier)

Product Datasheet
COA
SDS

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319-FG/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

319-FG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.

319-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-17

FGF‑17 is a member of the fibroblast growth factor (FGF) family. FGFs play multiple roles in biological functions, including angiogenesis, mitogenesis, cell differentiation and wound repair. FGFs share 30‑70% amino acid (aa) identity in a conserved, approximately 120 amino acid core domain (1‑3). The human or mouse FGF‑17 cDNA encodes a cleavable 22 aa signal sequence and a 194 secreted mature protein (1). Mature human FGF‑17 shares 99% aa identity with mouse, rat, porcine and canine FGF‑17. The FGF domain of FGF‑17 shares the most aa identity with FGF‑8 (~75%) and FGF‑18 (~64%). These three FGFs constitute a subfamily that overlaps in some areas of expression and function (1‑5). All are reported to bind and signal through FGF R4 the “c” splice forms of FGF R1‑3 (6, 7). During embryogenesis, FGF‑17 plays an organizing and inducing role in the patterning at the midbrain/hindbrain junction, and is also expressed in hindgut, parts of the developing skeleton, tail bud, major arteries, and heart (2‑5). In many of these areas, it is expressed along with FGF‑8, but slightly later (2‑6). Unlike FGF‑8 and FGF‑18, deletion of FGF‑17 produces viable mice. However, FGF‑17-/- mice show abnormalities in the dorsal frontal cortex, midbrain and cerebellum, manifested in some cases by ataxia, auditory defects, and abnormal social behavior (1, 4, 5, 8, 9). In humans, down‑regulation of FGF‑17 expression has been associated with Dandy‑Walker cerebellar malformation (10). FGF‑17 is also expressed in adult bovine ovarian follicles and the human prostate, and its expression is increased by both benign hypertrophy and cancer of the prostate (11‑13). FGF‑8, FGF‑17, and FGF‑18 are also abnormally expressed in many human leukemic cell lines and can enhance growth of cancer cells (14).

References
  1. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  2. Maruoka, Y. et al. (1998) Mech. Dev. 74:175.
  3. Xu, J. et al. (1999) Mech. Dev. 83:165.
  4. Cholfin, J.A. and J.L.R. Rubenstein (2007) Proc. Natl. Acad. Sci. USA 104:7652.
  5. Xu, J. et al. (2000) Development 127:1833.
  6. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
  7. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  8. Yu, X. et al. (2011) Neuroimage 56:1251.
  9. Scearce-Levie, K. et al. (2008) Genes Brain Behav. 7:344.
  10. Zanni, G. et al. (2011) Neurogenetics 12:241.
  11. Machado, M.F. et al. (2009) J. Endocrinol. 202:347.
  12. Polnaszek, N. et al. (2004) Prostate 60:18.
  13. Heer, R. et al. (2004) J. Pathol. 204:578.
  14. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.
Long Name
Fibroblast Growth Factor 17
Entrez Gene IDs
8822 (Human); 14171 (Mouse)
Alternate Names
FGF-13; FGF17; FGF-17; fibroblast growth factor 17

Citation for Recombinant Human FGF-17 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010-10-01;177(5):2379-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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