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Recombinant Human FGF-19 Protein

Catalog #: 969-FG
24 Citations Datasheet / COA / SDS

Carrier Free

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969-FG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
969-FG-025
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Citations (24)
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Recombinant Human FGF-19 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human FGF R4 Fc Chimera (Catalog # 685‑FR) is immobilized at 5 µg/mL (100 µL/well), the concentration range of Recombinant Human FGF-19 that produces 50% of the optimal binding response is  20-120 ng/mL.
Source
E. coli-derived human FGF-19 protein
Leu25-Lys216
Accession #
O95750
N-terminal Sequence
Analysis
Leu25
Predicted Molecular Mass
21 kDa

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969-FG (with carrier)

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969-FG/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

969-FG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

969-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-19

Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4).

Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites (1, 2). Among FGF family members, human FGF-19 is most closely related to chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively (1, 2, 5). Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues (1, 2). Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4 (2). Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2 (4). During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development (5).

References
  1. Nishimura, T. et al. (1999) Biocheim. Biophys. Acta 1444:148.
  2. Xie, M. et al. (1999) Cytokine 11:729.
  3. Goldfarb, M. (1996) Cytokine & Growth Factor Reviews 7:311.
  4. Green, P. et al. (1996) BioEssays 18:639.
  5. Ladher, R.K. et al. (2000) Science 290:1965.
Long Name
Fibroblast Growth Factor 19
Entrez Gene IDs
9965 (Human)
Alternate Names
FGF19; FGF-19; fibroblast growth factor 19

Citations for Recombinant Human FGF-19 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. PTX promotes breast cancer migration and invasion by recruiting ATF4 to upregulate FGF19
    Authors: Xue, T;Wang, X;Pan, X;Liu, M;Xu, F;
    Cellular signalling
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A New Approach to the Quantification of Fibroblast Growth Factor 23-An Array Surface Plasmon Resonance Imaging Biosensor
    Authors: Tokarzewicz, A;O?dak, ?;M?ynarczyk, G;Klekotka, U;Gorodkiewicz, E;
    International journal of molecular sciences
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  3. High level of polarized engraftment of porcine intrahepatic cholangiocyte organoids in decellularized liver scaffolds
    Authors: M Krüger, RA Samsom, LA Oosterhoff, ME van Wolfer, HS Kooistra, N Geijsen, LC Penning, LM Kock, P Sainz-Arna, PM Baptista, B Spee
    Journal of Cellular and Molecular Medicine, 2022-08-26;0(0):.
    Species: Porcine
    Sample Types: Organoids
    Applications: Organoid Culture
  4. Ileal FXR-FGF15/19 signaling activation improves skeletal muscle loss in aged mice
    Authors: Y Qiu, J Yu, X Ji, H Yu, M Xue, F Zhang, Y Li, Z Bao
    Mechanisms of Ageing and Development, 2022-01-10;202(0):111630.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Mechano-modulatory synthetic niches for liver organoid derivation
    Authors: G Sorrentino, S Rezakhani, E Yildiz, S Nuciforo, MH Heim, MP Lutolf, K Schoonjans
    Nat Commun, 2020-07-10;11(1):3416.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Long-Term Survival of Transplanted Autologous Canine Liver Organoids in a COMMD1-Deficient Dog Model of Metabolic Liver Disease
    Authors: HS Kruitwagen, LA Oosterhoff, ME van Wolfer, C Chen, S Nantasanti, K Schneeberg, A Kummeling, G van Strate, IC Akkerdaas, CR Vinke, FG van Steenb, LWL van Brugge, J Wolfswinke, GCM Grinwis, SA Fuchs, H Gehart, N Geijsen, RG Vries, H Clevers, J Rothuizen, BA Schotanus, LC Penning, B Spee
    Cells, 2020-02-11;9(2):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism
    Authors: F Huang, X Zheng, X Ma, R Jiang, W Zhou, S Zhou, Y Zhang, S Lei, S Wang, J Kuang, X Han, M Wei, Y You, M Li, Y Li, D Liang, J Liu, T Chen, C Yan, R Wei, C Rajani, C Shen, G Xie, Z Bian, H Li, A Zhao, W Jia
    Nat Commun, 2019-10-31;10(1):4971.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Reduced FXR Target Gene Expression in Copper-Laden Livers of COMMD1-Deficient Dogs
    Authors: X Wu, H Chien, ME van Wolfer, HS Kruitwagen, LA Oosterhoff, LC Penning
    Vet Sci, 2019-09-30;6(4):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Pre-clinical development of U3-1784, a novel FGFR4 antibody against cancer, and avoidance of its on-target toxicity
    Authors: R Bartz, K Fukuchi, T Ohtsuka, T Lange, K Gruner, I Watanabe, S Hayashi, Y Oda, R Kawaida, H Komori, Y Kashimoto, P Wirtz, JA Mayer, M Redondo-Mü, S Saito, M Takahashi, H Hanzawa, E Imai, A Martinez, M Hanai, D Häussinger, RW Chapman, T Agatsuma, J Bange, R Abraham
    Mol. Cancer Ther., 2019-07-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system
    Authors: D Garnier, R Li, F Delbos, A Fourrier, C Collet, C Guguen-Gui, C Chesné, TH Nguyen
    Sci Rep, 2018-05-29;8(1):8222.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  11. ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA
    Authors: YC Chen, CL Tsai, YH Wei, YT Wu, WT Hsu, HC Lin, YC Hsu
    Cell Death Dis, 2018-04-01;9(4):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Induction of differentiation of intrahepatic cholangiocarcinoma cells to functional hepatocytes using an organoid culture system
    Authors: Y Saito, T Nakaoka, T Muramatsu, H Ojima, A Sukeda, Y Sugiyama, R Uchida, R Furukawa, A Kitahara, T Sato, Y Kanai, H Saito
    Sci Rep, 2018-02-12;8(1):2821.
    Species: Human
    Sample Types: Organoids
    Applications: Bioassay
  13. Suppressor of Fused Controls Cerebellar Neuronal Differentiation in a Manner Modulated by GLI3 Repressor and Fgf15
    Authors: JJ Kim, T Jiwani, S Erwood, J Loree, ND Rosenblum
    Dev. Dyn., 2017-06-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. HER2-overexpressing breast cancers amplify FGFR signaling upon acquisition of resistance to dual therapeutic blockade of HER2
    Authors: AB Hanker, JG Garrett, MV Estrada, PD Moore, PG Ericsson, JP Koch, E Langley, S Singh, PS Kim, GM Frampton, EM Sanford, P Owns, J Becker, MR Groseclose, S Castellino, H Joensuu, J Huober, JC Brase, M Samira, S Brohée, D Venet, D Brown, J Baselga, M Piccart, C Sotiriou, CL Arteaga
    Clin. Cancer Res., 2017-04-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Quantitative liver proteomics identifies FGF19 targets that couple metabolism and proliferation
    Authors: V Massafra, A Milona, HR Vos, BM Burgering, SW van Mil
    PLoS ONE, 2017-02-08;12(2):e0171185.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Inner ear hair cell-like cells from human embryonic stem cells.
    Authors: Ronaghi, Mohammad, Nasr, Marjan, Ealy, Megan, Durruthy-Durruthy, Robert, Waldhaus, Joerg, Diaz, Giovanni, Joubert, Lydia-Ma, Oshima, Kazuo, Heller, Stefan
    Stem Cells Dev, 2014-03-10;23(11):1275-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Endocrine fibroblast growth factor FGF19 promotes prostate cancer progression.
    Authors: Feng S, Dakhova O, Creighton C, Ittmann M
    Cancer Res, 2013-02-25;73(8):2551-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
    Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
    Stem Cells, 2012-07-01;30(7):1455-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Activation of the farnesoid x receptor induces hepatic expression and secretion of fibroblast growth factor 21.
    Authors: Cyphert HA, Ge X, Kohan AB, Salati LM, Zhang Y, Hillgartner FB
    J. Biol. Chem., 2012-06-01;287(30):25123-38.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010-10-01;177(5):2379-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. The hepatic response to FGF19 is impaired in patients with nonalcoholic fatty liver disease and insulin resistance.
    Authors: Schreuder TC, Marsman HA, Lenicek M
    Am. J. Physiol. Gastrointest. Liver Physiol., 2010-01-21;298(3):G440-5.
    Applications: ELISA (Standard)
  22. High expression of the bile salt-homeostatic hormone fibroblast growth factor 19 in the liver of patients with extrahepatic cholestasis.
    Authors: Schaap FG, van der Gaag NA, Gouma DJ
    Hepatology, 2009-04-01;49(4):1228-35.
    Applications: ELISA (Standard)
  23. FGF15/FGFR4 integrates growth factor signaling with hepatic bile acid metabolism and insulin action.
    Authors: Shin DJ, Osborne TF
    J. Biol. Chem., 2009-02-23;284(17):11110-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc.
    Authors: Nakayama T, Mutsuga N, Tosato G
    Blood, 2006-10-31;109(4):1363-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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