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Recombinant Human FGF-20 Protein

Catalog #: 2547-FG
5 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
2547-FG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2547-FG-025
Graph showing dose-dependent bioactivity of Human FGF-20 protein
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Product Details
Citations (5)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF-20 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of heparin. The ED50 for this effect is 10.0-100 ng/mL.

Source
E. coli-derived human FGF-20 protein
Ala2-Thr211, with an N-terminal Met and a 6-His tag
Accession #
Q9NP95
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
24 kDa

Product Datasheets

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2547-FG (with carrier)

Product Datasheet
COA
SDS

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2547-FG/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2547-FG

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, (NH4)2SO4, DTT and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2547-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, (NH4)2SO4, DTT and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Graph showing dose-dependent bioactivity of Human FGF-20 protein View Larger

Recombinant Human FGF-20 Protein (Catalog # 2547-FG) stimulates proliferation of Balb/3T3 mouse embryonic fibroblast cells in the presence of heparin. The ED50 for this effect is 10.0-100 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-20

Fibroblast growth factor 20 (FGF-20) is a member of the FGF gene family, which currently contains 22 members. Based on its structure, it is further classified as an FGF-9 subfamily member. All FGF family members are heparin-binding growth factors with a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1). The cDNA of FGF-20 predicts a 211 aa polypeptide without a canonical signal peptide sequence, a feature shared with other members of this subfamily (2-4). Nevertheless, it is secreted with a molecular weight of 27 kDa (2-4). FGF-20 is known to bind to heparin (4). No alternate splice forms have been reported. However, three amino acid polymorphisms are known, and single nucleotide polymorphisms in noncoding regions that may effect expression show a strong correlation with a risk of developing Parkinson’s disease (5, 6). Human FGF-20 shows 98% aa identity to bovine FGF-20 and 95% aa identity to both rat and mouse FGF-20. Within the FGF-9 subfamily, FGF-20 is 69% and 63% aa identical to human FGF-9 and FGF-16, respectively. Human FGF-20 is reported to be promiscuous in its selection of receptors which include FGF R1c, FGF R2c, FGF R3b, FGF R3c and FGF R4 (4, 7, 8). FGF-20 is expressed a variety of cells, including dopaminergic neurons (2), fibroblasts, keratinocytes and breast epithelium (4), and multiple sites in the fetus (2, 7). Finally, the expression of FGF-20 and DKK-1 is regulated by beta -catenin during development and tumorigenesis, implying that FGF-20 may play a role in the oncogenesis induced by the Wnt signaling pathway (9).

References
  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Ohmachi, S. et al. (2000) Biochem. Biophys. Res. Commun. 277:355.
  3. Kirikoshi, H. et al. (2000) Biochem. Biophys. Res. Commun. 274:337.
  4. Jeffers, M. et al. (2001) Cancer Res. 61:3131.
  5. van der Walt, J. et al. (2004) Am. J. Hum. Genet. 74:1121.
  6. Genbank Accession # Q9NP95.
  7. Lavine, K.J. et al. (2005) Dev. Cell 8:85.
  8. Ohmachi, S. et al. (2003) J. Neurosci. Res. 72:436.
  9. Chamorro, M.N. et al. (2005) EMBO J. 24:73.
Long Name
Fibroblast Growth Factor 20
Entrez Gene IDs
26281 (Human)
Alternate Names
FGF20; FGF-20; fibroblast growth factor 20

Citations for Recombinant Human FGF-20 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The transcriptome of utricle hair cell regeneration in the avian inner ear.
    Authors: Ku Y, Renaud N, Veile R, Helms C, Voelker C, Warchol M, Lovett M
    J Neurosci, 2014-03-05;34(10):3523-35.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. Differential targeting of the dopamine transporter to recycling or degradative pathways during amphetamine- or PKC-regulated endocytosis in dopamine neurons.
    Authors: Hong, Weimin C, Amara, Susan G
    FASEB J, 2013-04-23;27(8):2995-3007.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010-10-01;177(5):2379-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Fgf20 is required for sensory epithelial specification in the developing cochlea.
    Authors: Hayashi T, Ray CA, Bermingham-McDonogh O
    J. Neurosci., 2008-06-04;28(23):5991-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Variation in the miRNA-433 binding site of FGF20 confers risk for Parkinson disease by overexpression of alpha-synuclein.
    Authors: Wang G, van der Walt JM, Mayhew G, Li YJ, Zuchner S, Scott WK, Martin ER, Vance JM
    Am. J. Hum. Genet., 2008-01-31;82(2):283-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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