Recombinant Human FGF-5 Protein Summary
Product Specifications
Glu23-Gly268 (Lys238Asn and Pro245Ser), with an N-terminal Met & Leu26-Gly268 (Lys238Asn and Pro245Ser)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
237-F5
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with Trehalose and with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing 1 μg/mL Sodium Heparin and at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
237-F5/CF
| Formulation | Supplied as a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: FGF-5
The FGF family is comprised of at least seven polypeptides that are potent regulators of cell proliferation, differentiation and function. All FGFs have two conserved cysteine residues and share 30 - 50% sequence homology at the amino acid level. FGF-5 was originally identified as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors. FGF-5 cDNA encodes a 267 amino acid residue protein with a putative 22 amino acid residue signal peptide. The murine homologue of FGF-5 was cloned and found to be 84% homologous to the human protein at the amino acid sequence level. Human and murine FGF-5 exhibit cross species activity.
In vitro, rhFGF-5 is a mitogen for Balb/3T3 fibroblasts and bovine heart endothelial cells. FGF-5 was also reported to be a major muscle-derived survival factor for cultured spinal motoneurons. In vivo, FGF-5 is suggested to play important roles in both embryology and neurobiology. Developmentally, FGF-5 mRNA is initially found in the embryoblast followed by the lateral somatic mesoderm, where it may play a role in angiogenesis, plus the myotomes cranial to the tail region, where it may delay terminal myoblast differentiation during cell migration. FGF-5 continues to impact muscle post-natally where it is believed to function as a target-derived neurotrophic factor of skeletal muscle. In the nervous system, FGF-5 has been most often identified in neurons associated with the limbic system, notably in neurons of the olfactory bulb and pyramidal cells of the hippocampus. Hippocampal FGF-5 is suggested to serve as a neurotrophic and differentiative factor for cholinergic and serotonergic neurons projecting to this region.
Citations for Recombinant Human FGF-5 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Macroscopic quorum sensing sustains differentiating embryonic stem cells
Authors: H Daneshpour, P van den Be, CH Chao, TG Fazzio, H Youk
Nature Chemical Biology, 2023-01-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Isoform-specific inhibition of FGFR signaling achieved by a de-novo-designed mini-protein
Authors: JS Park, J Choi, L Cao, J Mohanty, Y Suzuki, A Park, D Baker, J Schlessing, S Lee
Cell Reports, 2022-10-25;41(4):111545.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210
Authors: Y Tang, S Xiong, P Yu, F Liu, L Cheng
Cell Rep, 2018-07-31;24(5):1355-1362.e3.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
FGF5 is a crucial regulator of hair length in humans.
Authors: Higgins, Claire A, Petukhova, Lynn, Harel, Sivan, Ho, Yuan Y, Drill, Esther, Shapiro, Lawrence, Wajid, Muhammad, Christiano, Angela M
Proc Natl Acad Sci U S A, 2014-07-02;111(29):10648-53.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay -
Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
Stem Cells, 2012-07-01;30(7):1455-64.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
Authors: Tomlinson DC, Knowles MA
Am. J. Pathol., 2010-10-01;177(5):2379-86.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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