Recombinant Human FLRG Protein
Recombinant Human FLRG Protein Summary
Product Specifications
Met27-Val263, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1288-F3
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1288-F3/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Follistatin-related Gene Protein/FLRG
Follistatin-related gene protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Human FLRG cDNA encodes a 263 amino acid (aa) residue protein with a putative 26 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1 - 5).
- Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
- Sidis, Y. et al. (2002) Endocrinology 143:1613.
- Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
- Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
- Bartholin, L. et al. (2001) Oncogene 20:5409.
Citations for Recombinant Human FLRG Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
5
Citations: Showing 1 - 5
Filter your results:
Filter by:
-
Structural basis for potency differences between GDF8 and GDF11
Authors: RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson
BMC Biol, 2017-03-03;15(1):19.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Prediction of gestational diabetes mellitus by maternal factors and biomarkers at 11 to 13 weeks.
Authors: Nanda S, Savvidou M, Syngelaki A, Akolekar R, Nicolaides KH
Prenat. Diagn., 2010-12-28;31(2):135-41.
Applications: ELISA (Standard) -
Maternal plasma levels of follistatin-related gene protein in the first trimester of pregnancies with Down syndrome.
Authors: Miron P, Lambert J, Marcil A, Cowans NJ, Stamatopoulou A, Spencer K
Prenat. Diagn., 2010-03-01;30(3):224-8.
Applications: ELISA (Standard) -
BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
Blood, 2008-07-29;112(8):3154-63.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding.
Authors: Butler GS, Dean RA, Tam EM, Overall CM
Mol. Cell. Biol., 2008-05-27;28(15):4896-914.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human FLRG Protein
There are currently no reviews for this product. Be the first to review Recombinant Human FLRG Protein and earn rewards!
Have you used Recombinant Human FLRG Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image