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Recombinant Human Frizzled-7 Fc Chimera Protein, CF

Catalog #: 6178-FZ
4 Citations 1 Review Datasheet / COA / SDS
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6178-FZ-050
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Citations (4)
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Frizzled-7 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-7/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-7/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Frizzled-7 protein
Human Frizzled-7
(Gln33-Leu185)
Accession # NP_003498
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
NP_003498
N-terminal Sequence
Analysis
Gln33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43.3 kDa (monomer)
SDS-PAGE
55-66 kDa, reducing conditions

Product Datasheets

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6178-FZ

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6178-FZ

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Frizzled-7

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1 - 3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N-terminal signal peptide (amino acid (aa) 1 - 32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44 - 169), a variable-length linker region (aa 170 ‑ 256), a seven-pass transmembrane region (aa 257 - 549), and a variable-length C-terminal cytoplasmic domain (aa 550 - 574) (1 - 3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33 - 185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta -Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3 - 5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).

References
  1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
  2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.
  3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  4. Medina, A. et al. (2000) Mech. Dev. 92:227.
  5. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  6. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
  7. Melchior, K. et al. (2008) Biol. Chem. 389:897.
  8. Vincan, E. et al. (2007) Oncogene 26:2340.
Entrez Gene IDs
8324 (Human); 14369 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; fz-7; FZD7; FzE3frizzled-7; hFz7

Citations for Recombinant Human Frizzled-7 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy
    Authors: R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers
    Embo Molecular Medicine, 2021-06-09;0(0):e13977.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Regulation of ABCG2 expression by Wnt5a through FZD7 in human pancreatic cancer cells
    Authors: Z Zhang, S Gao, Y Xu, C Zhao
    Mol Med Rep, 2020-11-17;23(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Microglia-induced activation of non-canonical Wnt signaling aggravates neurodegeneration in demyelinating disorders
    Mol Cell Biol, 2016-08-22;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Frizzled-7 Fc Chimera Protein, CF
By Anonymous on 02/26/2018
Application: Binding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Human Frizzled-7 Fc Chimera Protein, CF 6178-FZ