Recombinant Human Frizzled-9 Fc Chimera Protein, CF Summary
Product Specifications
| Human Frizzled-9 (Leu23-Asp185) Accession # O00144 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9175-FZ
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Frizzled-9
Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mature human Frizzled-9 consists of a 207 amino acid (aa) N-terminal extracellular domain (ECD), the 7TM region, and a 62 aa C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region that binds Wnts and is highly conserved among Frizzleds. Within aa 23-185 of the N-terminal ECD, human Frizzled-9 shares 97% aa identity with mouse and rat Frizzled-9. The human Frizzled-9 gene maps to a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the central nervous system and myotomes during development, and it persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, differentiating osteoblasts, and developing myotubes of skeletal muscle (4-7). Mice lacking Frizzled-9 exhibit defects in learning, B lymphocyte development, and bone mineralization (4-6, 8). Frizzled-9 can signal through both canonical and non-canonical pathways (6, 9). Its binding to Wnt-7a promotes the activation of PPAR gamma, expression of Cadherin and Sprouty, and reversal of non-small cell lung cancer cell transformation (10-12).
- Dijksterhuis, J.P. et al. (2014) Br. J. Pharmacol. 171:1195.
- Wang, Y.K. et al. (1997) Hum. Mol. Genet. 6:465.
- Wang, Y.K. et al. (1999) Genomics 57:235.
- Zhao, C. et al. (2005) Development 132:2917.
- Ranheim, E.A. et al. (2005) Blood 105:2487.
- Albers, J. et al. (2011) J. Cell Biol. 192:1057.
- Aviles, E.C. et al. (2014) Front. Cell. Neurosci. 8:110.
- Heilmann, A. et al. (2013) PLoS One 8:e84232.
- Karasawa, T. et al. (2002) J. Biol. Chem. 277:37479.
- Winn, R.A. et al. (2005) J. Biol. Chem. 280:19625.
- Winn, R.A. et al. (2006) J. Biol. Chem. 281:26943.
- Tennis, M.A. et al. (2010) Mol. Cancer Res. 8:833.
Citation for Recombinant Human Frizzled-9 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy
Authors: R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers
Embo Molecular Medicine, 2021-06-09;0(0):e13977.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
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