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Recombinant Human FSH alpha/beta Protein

Catalog #: 5925-FS
12 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
5925-FS-010/CF

With Carrier

Catalog # Availability Size / Price Qty
5925-FS-010
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Recombinant Human FSH alpha/beta Protein Bioactivity
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Product Details
Citations (12)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FSH alpha/beta Protein Summary

Product Specifications

Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human FSH R. The ED50 for this effect is 25‑200 pg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived FSH alpha/beta protein
Human FSH alpha  
Ala25-Ser116
Accession # P01215
Human FSH beta
Cys21-Glu129
Accession # P01225
N-terminus C-terminus
Accession #
P01215 (FSH alpha ) & P01225 (FSH beta )
N-terminal Sequence
Analysis
Ala25 (FSH alpha ) & Cys21 (FSH beta )
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
10.2 kDa (FSH alpha ) & 12.4 kDa (FSH beta )
SDS-PAGE
22-26 kDa, reducing conditions

Product Datasheets

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5925-FS (with carrier)

Product Datasheet
COA
SDS

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5925-FS/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5925-FS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5925-FS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human FSH alpha/beta Protein Bioactivity View Larger

Recombinant Human FSH alpha / beta (Catalog # 5925-FS) induces cAMP accumulation in the HEK293 human embryonic kidney cell line transfected with human FSH R. The ED50 for this effect is 25-200 pg/mL.

SDS-PAGE Recombinant Human FSH alpha/beta Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Human FSH alpha / beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 20-25 kDa.

Gel Filtration Chromatography Recombinant Human FSH alpha/beta Protein Gel Filtration Chromatography View Larger

40 µg of Recombinant Human FSH alpha / beta (blue line) was applied onto a Superdex 200 column. The peak eluted at approximately 44 kDa, form overlaid on the molecular weight markers (gray line), corresponds to the dimeric molecular mass.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FSH alpha/beta

Follicle-Stimulating Hormone (FSH) is a 33 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit is also a component of Luteinizing Hormone (LH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with the FSH Receptor (1-3). The approximately 20 kDa alpha subunit of human FSH shares 73% and 72% aa sequence identity with the mouse and rat orthologs, respectively. The approximately 21 kDa human FSH beta subunit shares 85% and 82% aa sequence identity with the mouse and rat orthologs, respectively. FSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, FSH secretion can also be stimulated by activins and inhibited by inhibins (4-6). FSH works in concert with LH to regulate female reproduction; FSH stimulates follicular growth and LH induces ovulation (7). FSH can also regulate estrogen formation by inducing the aromatase enzyme that converts androgen to estradiol (7, 8). In addition, it has been suggested to have a role in postmenopausal bone loss and in the neovascularization surrounding malignant tumors (9-11). In males, FSH is critical for sperm production (12). Mutations in the human FSH and FSHR genes are associated with reduced reproductive functioning in females (2, 11). Additionally, increased production of autoantibodies directed against FSH beta has been suggested to contribute to certain types of female infertility (2). Assisted reproductive technology commonly uses recombinant FSH to induce follicle growth (7).

References
  1. Fox, K.M. et al. (2001) Mol. Endocrinol. 15:378.
  2. Haller-Kikkatalo, K. et al. (2012) Clin. Dev. Immunol. 2012:762541.
  3. Jiang, X. et al. (2014) Mol. Cell. Endocrinol. 382:424.
  4. Findlay, J.K. (1993) Biol. Reprod. 48:15.
  5. Marshall, J.C. et al. (1993) Trans. Am. Clin. Climatol. Assoc. 104:31.
  6. Bernard, D.J. and S. Tran (2013) Biol. Reprod. 88:1.
  7. Raju, G.A. et al. (2013) J. Hum. Reprod. Sci. 6:227.
  8. Erickson, G.F. and A.J. Hsueh (1978) Endocrinology 102:1275.
  9. Sun, L. et al. (2006) Cell 125:247.
  10. Radu, A. et al. (2010) N. Engl. J. Med. 363:1621.
  11. Laan, M. et al. (2012) Curr. Opin. Endocrinol. Diabetes Obes. 19:220.
  12. Franchimont, P. et al. (1975) J. Reprod. Fertil. 44:335.
Long Name
Follicle Stimulating Hormone
Entrez Gene IDs
2488 (Human); 14308 (Mouse); 25447 (Rat)
Alternate Names
FSH alpha/beta

Citations for Recombinant Human FSH alpha/beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Pigment epithelium-derived factor expression and role in follicular development
    Authors: Tarabeih, R;Nemerovsky, L;Bar-Joseph, H;Eldar-Boock, A;Elmechaly, CL;Ben-Ami, I;Shalgi, R;
    Reproductive biomedicine online
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. High FSH levels impair VEGF secretion of human, frozen-thawed ovarian cortical tissue in vitro
    Authors: Einenkel, R;Schallmoser, A;Sänger, N;
    Scientific reports
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  3. Phosphoproteomic analysis on ovarian follicles reveals the involvement of LSD1 phosphorylation in Chicken follicle selection
    Authors: Y Zhang, Q Chen, Y Guo, L Kang, Y Sun, Y Jiang
    BMC Genomics, 2023-03-13;24(1):109.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Intra-pituitary follicle-stimulating hormone signaling regulates hepatic lipid metabolism in mice
    Authors: S Qiao, S Alasmi, A Wyatt, P Wartenberg, H Wang, M Candlish, D Das, M Aoki, R Grünewald, Z Zhou, Q Tian, Q Yu, V Götz, A Belkacemi, A Raza, F Ectors, K Kattler, G Gasparoni, J Walter, P Lipp, P Mollard, DJ Bernard, E Karatayli, SC Karatayli, F Lammert, U Boehm
    Nature Communications, 2023-02-25;14(1):1098.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch
    Authors: FL Zhang, YQ Feng, JY Wang, KX Zhu, L Wang, JM Yan, XX Li, JJ Wang, W Ge, M De Felici, W Shen
    Cell Death & Disease, 2023-02-17;14(2):134.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  6. 1-Nitropyrene exposure induces mitochondria dysfunction and impairs oocyte maturation in mice
    Authors: X Yu, F Meng, J Huang, W Li, J Zhang, S Yin, L Zhang, S Wang
    Ecotoxicology and environmental safety, 2022-07-28;242(0):113921.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Stimulation
  7. Transcriptomic and proteomic analyses of ovarian follicles reveal the role of VLDLR in chicken follicle selection
    Authors: Q Chen, Y Wang, Z Liu, X Guo, Y Sun, L Kang, Y Jiang
    BMC Genomics, 2020-07-16;21(1):486.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Growth differentiation factor 9 promotes follicle-stimulating hormone-induced progesterone production in chicken follicular granulosa cells
    Authors: J Li, W Luo, T Huang, Y Gong
    Gen. Comp. Endocrinol., 2019-03-06;0(0):.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer
    Authors: W Marlicz, A Poniewiers, S Rzeszotek, R Bartoszews, K Skonieczna, T Starzy?ska, MZ Ratajczak
    PLoS ONE, 2018-03-01;13(3):e0189337.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Macromolecular crowded conditions strengthen contacts between mouse oocytes and companion granulosa cells during in vitro growth
    Authors: S Mizumachi, T Aritomi, K Sasaki, K Matsubara, Y Hirao
    J. Reprod. Dev., 2018-02-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  11. In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells
    Authors: D Jung, J Xiong, M Ye, X Qin, L Li, S Cheng, M Luo, J Peng, J Dong, F Tang, W Shen, MM Matzuk, K Kee
    Nat Commun, 2017-06-12;8(0):15680.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Conserved miR-10 family represses proliferation and induces apoptosis in ovarian granulosa cells
    Authors: T Jiajie, Y Yanzhou, AC Hoi-Hung, C Zi-Jiang, C Wai-Yee
    Sci Rep, 2017-01-23;7(0):41304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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