Recombinant Human Galectin-8 Protein Summary
Product Specifications
Met1-Trp317
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1305-GA
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG 8000 and Trehalose with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1305-GA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG 8000 and Trehalose. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Galectin-8
The galectins constitute a large family of carbohydrate-binding proteins with specificity for N‑acetyl‑lactosamine‑containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (‑1, ‑2, ‑5, ‑7, ‑10, ‑11, ‑13, ‑14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain, and the tandem‑repeat Galectins (‑4, ‑6, ‑8, ‑9, ‑12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).
Galectin-8, also known as prostate carcinoma tumor antigen 1 (PCTA1) in human, is a tandem repeat‑type galectin. Prototype (single CRD) isoforms arising through alternate gene splicing have also been identified (5). Galectin-8 is highly expressed in lung carcinomas, certain forms of prostate carcinomas, as well as other tumor cells. It binds to a subset of cell surface integrins to modulate ECM‑integrin interactions. As a soluble ligand, Galectin-8 can inhibit cell adhesion (6). Immobilized Galectin-8, however, has also been shown to promote cell adhesion (7). Human and mouse Galectin-8 share approximately 80% amino acid homology (4).
- Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
- Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
- Hughes, R.C. (2002) Biochimie 83:667.
- R&D Systems’ Cytokine Bulletin, Summer, 2002.
- Bidon, N. et al. (2001) Gene 274:253.
- Hadari, Y. et al. (1995) J. Biol. Chem. 270:3447.
- Levy, Y. et al. (2001) J. Biol. Chem. 276:31285.
Citations for Recombinant Human Galectin-8 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Galectin-8 Contributes to Human Trophoblast Cell Invasion
Authors: Legner, J;Jovanovi? Krivoku?a, M;Viloti?, A;Pirkovi?, A;Nacka-Aleksi?, M;Boji?-Trbojevi?, ;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Computational repurposing of therapeutic small molecules from cancer to pulmonary hypertension
Authors: V Negi, J Yang, G Speyer, A Pulgarin, A Handen, J Zhao, YY Tai, Y Tang, MK Culley, Q Yu, P Forsythe, A Gorelova, AM Watson, Y Al Aaraj, T Satoh, M Sharifi-Sa, A Rajaratnam, J Sembrat, S Provencher, X Yin, SO Vargas, M Rojas, S Bonnet, S Torrino, BK Wagner, SL Schreiber, M Dai, T Bertero, I Al Ghouleh, S Kim, SY Chan
Science Advances, 2021-10-20;7(43):eabh3794.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human trophoblast requires galectin-3 for cell migration and invasion
Authors: Ž Boji?-Trbo, M Jovanovi?, A Viloti?, N Kolundži?, I Stefanoska, F Zetterberg, UJ Nilsson, H Leffler, L Vi?ovac
Sci Rep, 2019-02-14;9(1):2136.
Species: Human
Sample Types: Basement Membrane Extract
Applications: Bioassay -
Impact of Exogenous Galectin-9 on Human T Cells: CONTRIBUTION OF THE T CELL RECEPTOR COMPLEX TO ANTIGEN-INDEPENDENT ACTIVATION BUT NOT TO APOPTOSIS INDUCTION.
Authors: Lhuillier C, Barjon C, Niki T, Gelin A, Praz F, Morales O, Souquere S, Hirashima M, Wei M, Dellis O, Busson P
J Biol Chem, 2015-05-06;290(27):16797-811.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions.
Authors: Cueni LN, Detmar M
Exp. Cell Res., 2009-03-04;315(10):1715-23.
Species: Human
Sample Types:
Applications: Binding Assay
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The protein is in an oligomeric form, therefore a lot of boiling is required before loading on western gel as a control. The R&D antibody was used to probe for this protein.
