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Recombinant Human Gas6 Protein, CF

Catalog #: 885-GS
17 Citations Datasheet / COA / SDS
Newer Version Available: 885-GSB
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Discontinued Product

885-GS has been discontinued and is replaced by 885-GSB.

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Citations (17)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Gas6 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 25-150 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Gas6 protein
Ala49-Ala678, with a C-terminal 6-His tag
Accession #
NP_000811
N-terminal Sequence
Analysis
Ala49
Predicted Molecular Mass
70.5 kDa
SDS-PAGE
70-85 kDa, reducing conditions

Product Datasheets

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885-GS

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

885-GS

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Citrate.
Reconstitution Reconstitute at 100 μg/mL in sterile H2O.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is up‑regulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K‑dependent and have an extensively gamma ‑carboxylated N‑terminal Gla domain, four EGF‑like repeats, and a C‑terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77%‑79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the
EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3‑5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet‑mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF‑induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma ‑carboxylation of the Gla domain (12, 16).

References
  1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Stitt, T.N. et al. (1995) Cell 80:661.
  4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
  5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
  7. Sather, S. et al. (2007) Blood 109:1026.
  8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
  9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
  10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
  11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
  13. Gallicchio, M. et al. (2005) Blood 105:1970.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
  16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
Long Name
Growth-arrest-specific Protein 6
Entrez Gene IDs
2621 (Human); 14456 (Mouse)
Alternate Names
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

Citations for Recombinant Human Gas6 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. S100A10 is a critical mediator of GAS6/AXL-induced angiogenesis in renal cell carcinoma
    Authors: Y Xiao, H Zhao, L Tian, R Nolley, AN Diep, A Ernst, KC Fuh, YR Miao, R von Eyben, JT Leppert, JD Brooks, DM Peehl, AJ Giaccia, EB Rankin
    Cancer Res., 2019-10-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Inactivating mutations and X-ray crystal structure of the tumor suppressor OPCML reveal cancer-associated functions
    Authors: JR Birtley, M Alomary, E Zanini, J Antony, Z Maben, GC Weaver, C Von Arx, M Mura, AT Marinho, H Lu, EVN Morecroft, E Karali, NE Chayen, EW Tate, M Jurewicz, LJ Stern, C Recchi, H Gabra
    Nat Commun, 2019-07-17;10(1):3134.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs
    Authors: CM Ramsden, B Nommiste, A R Lane, AF Carr, MB Powner, M J K Smart, LL Chen, MN Muthiah, AR Webster, AT Moore, ME Cheetham, L da Cruz, PJ Coffey
    Sci Rep, 2017-03-03;7(1):51.
    Species: Ovine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Mutation of androgen receptor N-terminal phosphorylation site Tyr-267 leads to inhibition of nuclear translocation and DNA binding.
    Authors: Karaca M, Liu Y, Zhang Z, De Silva D, Parker J, Earp H, Whang Y
    PLoS ONE, 2015-05-07;10(5):e0126270.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. EGFRvIII-mediated transactivation of receptor tyrosine kinases in glioma: mechanism and therapeutic implications.
    Authors: Greenall S, Donoghue J, Van Sinderen M, Dubljevic V, Budiman S, Devlin M, Street I, Adams T, Johns T
    Oncogene, 2015-02-09;34(41):5277-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Inhibition of Axl improves the targeted therapy against ALK-mutated neuroblastoma.
    Authors: Xu F, Li H, Sun Y
    Biochem Biophys Res Commun, 2014-10-30;454(4):566-571.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells.
    Authors: Paolino, Magdalen, Choidas, Axel, Wallner, Stephani, Pranjic, Blanka, Uribesalgo, Iris, Loeser, Stefanie, Jamieson, Amanda M, Langdon, Wallace, Ikeda, Fumiyo, Fededa, Juan Pab, Cronin, Shane J, Nitsch, Roberto, Schultz-Fademrecht, Carsten, Eickhoff, Jan, Menninger, Sascha, Unger, Anke, Torka, Robert, Gruber, Thomas, Hinterleitner, Reinhard, Baier, Gottfrie, Wolf, Dominik, Ullrich, Axel, Klebl, Bert M, Penninger, Josef M
    Nature, 2014-02-19;507(7493):508-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Gas6 stimulates angiogenesis of human retinal endothelial cells and of zebrafish embryos via ERK1/2 signaling.
    Authors: Kim, Young So, Jung, Seung-Hy, Jung, Dong Ho, Choi, So-Jin, Lee, Yu-Ri, Kim, Jin Sook
    PLoS ONE, 2014-01-07;9(1):e83901.
    Species: Zebrafish
    Sample Types: Whole Organism
    Applications: In Vivo
  9. AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.
    Authors: Dunne, Philip D, McArt, Darragh, Blayney, Jaine K, Kalimutho, Murugan, Greer, Samanda, Wang, Tingting, Srivastava, Supriya, Ong, Chee Wee, Arthur, Ken, Loughrey, Maurice, Redmond, Keara, Longley, Daniel B, Salto-Tellez, Manuel, Johnston, Patrick, Van Schaeybroeck, Sandra
    Clin Cancer Res, 2013-10-29;20(1):164-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
    Authors: Zizzo G, Cohen P
    J Immunol, 2013-04-17;190(10):5237-46.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  11. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012-10-15;209(11):2033-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Targeting Axl with an high-affinity inhibitory aptamer.
    Authors: Cerchia, Laura, Esposito, Carla L, Camorani, Simona, Rienzo, Anna, Stasio, Loredana, Insabato, Luigi, Affuso, Andrea, de Franciscis, Vittorio
    Mol Ther, 2012-08-21;20(12):2291-303.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Axl is essential for VEGF-A-dependent activation of PI3K/Akt.
    Authors: Ruan GX, Kazlauskas A
    EMBO J., 2012-02-10;31(7):1692-703.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Human cutaneous melanomas lacking MITF and melanocyte differentiation antigens express a functional Axl receptor kinase.
    Authors: Sensi M, Catani M, Castellano G
    J. Invest. Dermatol., 2011-07-28;131(12):2448-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.
    Authors: Hung YJ, Lee CH, Chu NF
    Diabetes Care, 2010-05-26;33(8):1840-4.
    Applications: ELISA (Standard)
  16. Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.
    Authors: Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
    J. Immunol., 2009-08-05;183(5):3004-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Gas6 evaluation in patients with acute dyspnea due to suspected pulmonary embolism.
    Authors: Sainaghi PP, Alciato F, Carnieletto S, Castello L, Bergamasco L, Sola D, Bongo AS, Inglese E, Polosa R, Avanzi GC
    Respir Med, 2008-11-25;103(4):589-94.
    Applications: ELISA (Standard)

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