Recombinant Human GASP-1/WFIKKN2 Protein Summary
Product Specifications
Leu35-His576, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2070-GS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2070-GS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: GASP-1/WFIKKN2
Growth and differentiation factor-associated serum protein-1 (GASP-1) was isolated in a screen to identify proteins in mice that copurify with myostatin (GDF-8), a potent negative regulator of skeletal muscle mass (1). The GASP-1 cDNA encodes a 571 amino acid protein that contains a 29 amino acid signal sequence and is comprised of many conserved domains: WAP, Follistatin/Kazal, Immunoglobulin, two tandem Kunitz, and Netrin (1). Two related human proteins which contain the same domain structure are called WFIKKN (based on the presence and order of these conserved domains) and WFIKKNRP (WFIKKN-related protein) (2). Mouse GASP-1 is homologous to human WFIKKN2, also know as WFIKKNRP and mouse GASP-2 to human WFIKKN. Human GASP-1 is the same protein as WFIKKN2. WAP, Follistatin, Kazal and Netrin domains are all implicated in protease inhibition, and these proteins may be multivalent protease inhibitors (3). GASP-1 and -2 show distinct expression patterns both in the developing fetus and the adult. In the developing fetus, GASP-1 expression is highest in the brain, skeletal muscle, thymus and kidney while GASP-2 is abundant in the lung, skeletal muscle and liver (4). In the adult, GASP-1 is primarily expressed in the ovary, testis, and brain while GASP-2 is in the pancreas, liver, and thymus (4). GASP-1 inhibits myostatin and the highly related protein, GDF-11, but not Activin or TGF-beta in vitro (1). In addition, GASP-1 binds directly but independently to both mature myostatin and the myostatin propeptide (1). By amino acid sequence, human GASP-1 is 90% and 55% identical to mouse GASP-1 and human GASP-2, respectively.
- Hill, J.J. et al. (2003) Mol. Endo. 17:1144.
- Trexler, M. et al. (2001) Proc. Natl. Acad. Sci. 98:3705.
- Nagy, A. et al. (2003) Eur. Jour. Biochem. 270:2101.
- Trexler, M. et al. (2002) Biol. Chem. 383:223.
Citations for Recombinant Human GASP-1/WFIKKN2 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
WFIKKN2 is a bifunctional axon guidance cue that signals through divergent DCC family receptors
Authors: Nickerson, KR;Tom, I;Cortés, E;Abolafia, JR;Özkan, E;Gonzalez, LC;Jaworski, A;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Structural basis for potency differences between GDF8 and GDF11
Authors: RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson
BMC Biol, 2017-03-03;15(1):19.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Peroxisome proliferator-activated receptor beta/delta induces myogenesis by modulating myostatin activity.
Authors: Bonala S, Lokireddy S, Arigela H, Teng S, Wahli W, Sharma M, McFarlane C, Kambadur R
J. Biol. Chem., 2012-02-23;287(16):12935-51.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human GASP-1/WFIKKN2 Protein
There are currently no reviews for this product. Be the first to review Recombinant Human GASP-1/WFIKKN2 Protein and earn rewards!
Have you used Recombinant Human GASP-1/WFIKKN2 Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
