Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

Name: Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)
Brand: R&D Systems
SKU: 6987-GL
Rating: 4 (1 reviews)

Catalog #: 6987-GL
1 Citation 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
6987-GL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
6987-GL-025

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Product Details

Citations (1)

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Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed) Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human GITR. Wyzgol, A. et al. (2009) J. Immunol. 183:1851. The ED₅₀ for this effect is 4-20 ng/mL. The activity can be enhanced approximately 10-fold in the presence of
Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).

Source

Chinese Hamster Ovary cell line, CHO-derived human GITR Ligand/TNFSF18 protein

Hemagglutinin Tag (YPYDVPDYA)	GCN4-IZ	GGGSGGGSGGGS	Human GITR Ligand (Glu52-Ser177) Accession # Q9UNG2.3
N-terminus			C-terminus

Accession #

Q9UNG2.3

N-terminal Sequence
Analysis

Tyr

Structure / Form

Non-covalently-linked trimer

Predicted Molecular Mass

19.6 kDa (monomer)

SDS-PAGE

21-30 kDa, reducing conditions

Product Datasheets

6987-GL (with carrier)

Product Datasheet

COA

SDS

6987-GL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6987-GL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6987-GL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: GITR Ligand/TNFSF18

GITR Ligand, also known as TNFSF18 and TL6, is an approximately 30 kDa type II transmembrane glycoprotein in the TNF superfamily (1). Human GITR Ligand consists of a 50 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 128 aa extracellular domain (ECD) (2, 3). Within the ECD, human GITR Ligand shares 56% and 60% aa sequence identity with mouse and rat GITR Ligand, respectively. GITR Ligand is expressed on antigen presenting cells,
CD4^-CD8^- double negative thymic precursors, vascular endothelial cells, neurons, and in the eye (4-11). Its expression is transiently up‑regulated by proinflammatory stimulation (4, 8, 11). The binding of GITR Ligand to GITR on mouse CD25⁺ Treg cells permits the reactivation of T cells from Treg-induced suppression, although this does not appear to occur in humans (5, 9, 12-14). GITR Ligand binding to GITR additionally provides a co‑stimulatory signal to activated CD4⁺ and CD8⁺ T cells and NK cells (5, 6, 15, 16). This interaction also induces reverse signaling in GITR Ligand expressing dendritic cells to suppress cellular activation through the same pathway induced by the immunosuppressant dexamethasone (17). In the brain, GITR Ligand/GITR interactions enhance NGF-mediated neurite outgrowth from sympathetic neurons (10).

References

Azuma, M. (2010) Crit. Rev. Immunol. 30:547.
Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
Tone, M. et al. (2003) Proc. Natl. Acad. Sci. 100:15059.
Hanabuchi, S. et al. (2006) Blood 107:3617.
Kamimura, Y. et al. (2009) J. Immunol. 182:2708.
Hwang, H. et al. (2010) J. Neurosci. Res. 88:2188.
Tuyaerts, S. et al. (2007) J. Leukoc. Biol. 82:93.
O’Keefe, G.W. et al. (2008) Nat. Neurosci. 11:135.
Kim, B.J. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:3170.
Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
Ji, H. et al. (2004) J. Immunol. 172:5823.
McHugh, R.S. et al. (2002) Immunity 16:311.
Kanamaru, F. et al. (2004) J. Immunol. 172:7306.
Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
Grohmann, U. et al. (2007) Nat. Med. 13:579.

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene Ligand

Entrez Gene IDs

8995 (Human); 240873 (Mouse); 364031 (Rat)

Alternate Names

Activation-inducible TNF-related ligand; AITRL; AITRLMGC138237; GITR Ligand; GITRL; GITRLglucocorticoid-induced TNFR-related protein ligand; Glucocorticoid-induced TNF-related ligand; hGITRLGITR ligand; TL6AITR ligand; TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18

Related Research Areas

Citation for Recombinant Human GITR Ligand/TNFSF18 (CHO-expressed)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
MAbs, 2018-03-05;0(0):1-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR