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Recombinant Human GLP-1R Fc Chimera Protein, CF

Catalog #: 10646-GL Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10646-GL-050
Recombinant Human GLP-1R Fc Chimera Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GLP-1R Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When GLP-1 peptide (Tocris (Catalog # 5374) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GLP-1 Fc Chimera binds with an ED50 of 2-12 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human GLP-1R protein
Human GLP-1R
(Ala21-Glu139)
Accession # P43220.2		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
P43220.2
N-terminal Sequence
Analysis
Ala21 & Arg24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa
SDS-PAGE
60-72 kDa, under reducing conditions

Product Datasheets

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10646-GL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10646-GL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When GLP-1 peptide (5374) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GLP-1R Fc Chimera (Catalog # 10646-GL) binds with an ED50 of 2-12 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human GLP-1R Fc Chimera (Catalog # 10646-GL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-72 kDa and 120-144 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GLP-1R

Glucagon-like Peptide 1 Receptor (GLP-1R) is a member of the Class B or secretin receptor-like family of G protein-coupled receptors (GPCRs) (1, 2). Activation of GLP-1R signaling regulates secretion of insulin from pancreatic beta -cells in a glucose-dependent manner (3). Human GLP-1R consists of an N-terminal, extracellular domain (ECD) containing 6 conserved cysteine residues and a transmembrane domain with seven membrane-spanning alpha-helices, characteristic of GPCRs (2,4). The mature ECD of human GLP-1R shares 92% amino acid (aa) identity with mouse GLP-1R. GLP-1R is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach and it is a receptor for Glucagon-like Peptide, a regulator of insulin secretion by pancreatic beta cells (1, 4, 5). GLP1R agonists, including GLP-1, Boc5, and Cpd1, have been targeted for research and used as type 2 diabetes treatments (5, 6). GLP1R is also expressed in the brain where it is involved in the control of appetite (5).

References
  1. Song, G. et al. (2017) Nature. 546:312.
  2. Hollenstein, K. et al. (2013) Trends Pharmacol Sci. 35:12.
  3. Drucker, D.J. et al. (1987) PNAS. 84 (10):3434.
  4. Donnelly, D. (2012) Br. J. Pharmacol. 166:27.
  5. Simo, R. and Hernandez, C. (2017) Diabetes. 66:1453.
  6. Holst, J.J. (2004) Expert Opinion on Emerging Drugs. 9:155.

Long Name
Glucagon-like Peptide 1 Receptor
Entrez Gene IDs
2740 (Human); 14652 (Mouse)
Alternate Names
GLP-1 receptor; GLP1R; GLP-1R; GLP-1-R; glucagon-like peptide 1 receptor; MGC138331

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