Recombinant Human gp130 Fc Chimera Avi-tag Protein, CF

Recombinant Human gp130 Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: gp130

gp130 (glycoprotein 130, also known as IL-6RB and IL-6ST) is a subunit of receptor complexes for several cytokines that mediate highly diverse biological processes. These include functionally and structurally related cytokines within the IL-6 and IL-12 family including IL-6, IL-11, IL-27, IL-35, IL-39, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), novel neutrophin-1/B cell stimulating factor-3 or cardiotrophin-like cytokine (CLC) and neuropoetin (NP) (1, 2). gp130 is ubiquitously expressed in all cell types. Depending on the cytokine, binding of ligand leads to the formation of signalling complexes that trigger a sophisticated signalling machinery downstream of gp130. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R (3-6). Mature human CP130 consists of a 597 amino acid (aa) extracellular region (ECD), a 22 aa transmembrane domain, and a 277 aa cytoplasmic region. Within the ECD, human gp130 shares 73% and 75% aa sequence identity with mouse and rat gp130, respectively. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities. Our Avi-tag Biotinylated Recombinant Human gp130 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Related Research Areas

• Cytokines and Receptors in Allergy and Asthma
• Cytokines and Receptors in Angiogenesis
• Cytokines and Receptors in Tfh Cell Differentiation and Function
• Diabetes
• IL-12 Family
• IL-6 Family
• Myeloid-derived Suppressor Cells (MDSC)
• Obesity
• Receptors in the Jak/STAT Pathway
• T Cell Cytokine Signaling
• Th1 Cells
• Th17 Cells
• VPS10P-domain Family