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Recombinant Human gp130 Fc Chimera Protein, CF

Catalog #: 671-GP
11 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
671-GP-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (11)
FAQs
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human gp130 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED50 for this effect is 0.65-6.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human gp130 protein
Human gp130
(Glu23-Ile618)
Accession # P40189		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
P40189
N-terminal Sequence
Analysis
Glu23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
95 kDa (monomer)
SDS-PAGE
115-130 kDa, reducing conditions

Product Datasheets

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671-GP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

671-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage or the membrane-bound receptor of from alternative splicing, has been detected in human serum. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References
  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

Citations for Recombinant Human gp130 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Targeting IL-6 trans-signalling by sgp130Fc attenuates severity in SARS-CoV-2 -infected mice and reduces endotheliopathy
    Authors: Rodríguez-Hernández, MÁ;Baena-Bustos, M;Carneros, D;Zurita-Palomo, C;Muñoz-Pinillos, P;Millán, J;Padillo, FJ;Smerdou, C;von Kobbe, C;Rose-John, S;Bustos, M;
    EBioMedicine
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Variation in IL6ST cytokine family function and the potential of IL6 trans-signalling in ERalpha positive breast cancer cells
    Authors: D Mosly, K MacLeod, N Moir, A Turnbull, AH Sims, SP Langdon
    Cellular Signalling, 2022-12-21;103(0):110563.
    Species: Human
    Sample Types: cell culture
  3. Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells
    Authors: M Lindkvist, MM Zegeye, M Grenegård, LU Ljungberg
    International Journal of Molecular Sciences, 2022-01-27;23(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH
    Authors: J Dong, S Viswanatha, E Adami, BK Singh, SP Chothani, B Ng, WW Lim, J Zhou, M Tripathi, NSJ Ko, SG Shekeran, J Tan, SY Lim, M Wang, PM Lio, PM Yen, S Schafer, SA Cook, AA Widjaja
    Nature Communications, 2021-01-04;12(1):66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. RNA-Seq analysis reveals gene expression changes induced by IL-6 trans-signaling activation in retinal endothelial cells
    Authors: R Robinson, D Brown, L Churchwell, TJ Lee, SK Kodeboyina, J Bloom, A Sharma, S Sharma
    Cytokine, 2020-12-28;139(0):155375.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Interleukin-6 trans-signaling is a candidate mechanism to drive progression of human DCCs during clinical latency
    Authors: M Werner-Kle, A Grujovic, C Irlbeck, M Obradovi?, M Hoffmann, H Koerkel-Qu, X Lu, S Treitschke, C Köstler, C Botteron, K Weidele, C Werno, B Polzer, S Kirsch, M Gužvi?, J Warfsmann, K Honarnejad, Z Czyz, G Feliciello, I Blochberge, S Grunewald, E Schneider, G Haunschild, N Patwary, S Guetter, S Huber, B Rack, N Harbeck, S Buchholz, P Rümmele, N Heine, S Rose-John, CA Klein
    Nat Commun, 2020-10-05;11(1):4977.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Treatment of type 2 diabetes with the designer cytokine IC7Fc
    Authors: M Findeisen, TL Allen, DC Henstridge, H Kammoun, AE Brandon, LL Baggio, KI Watt, M Pal, L Cron, E Estevez, C Yang, GM Kowalski, L O'Reilly, C Egan, E Sun, LM Thai, G Krippner, TE Adams, RS Lee, J Grötzinger, C Garbers, S Risis, MJ Kraakman, NA Mellet, J Sligar, ET Kimber, RL Young, MA Cowley, CR Bruce, PJ Meikle, PA Baldock, P Gregorevic, TJ Biden, GJ Cooney, DJ Keating, DJ Drucker, S Rose-John, MA Febbraio
    Nature, 2019-09-25;574(7776):63-68.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  8. A soluble form of IL-27Ralpha is a natural IL-27 antagonist.
    Authors: Dietrich C, Candon S, Ruemmele F, Devergne O
    J Immunol, 2014-04-25;192(11):5382-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor.
    Authors: Chollangi, Srinivas, Mather, Timothy, Rodgers, Karla K, Ash, John D
    J Biol Chem, 2012-07-24;287(39):32848-59.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  10. Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
    Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
    Mol. Hum. Reprod., 2004-03-25;10(5):313-9.
    Applications: Control
  11. Interplay between IFN-gamma and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation.
    Authors: McLoughlin RM, Witowski J, Robson RL, Wilkinson TS, Hurst SM, Williams AS, Williams JD, Rose-John S, Jones SA, Topley N
    J. Clin. Invest., 2003-08-01;112(4):598-607.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human gp130 Fc Chimera Protein, CF
By Anonymous on 02/09/2018
Application: Characterization prior to use
Other Recombinant Human gp130 Fc Chimera Protein, CF 671-GP