Recombinant Human GST-KEAP1 Protein, CF

Recombinant Human GST-KEAP1 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Keap1

Kelch-like ECH-associated protein (KEAP1) is the substrate recognition subunit of a BCR (BTB-CUL3-RBX) E3 ubiquitin ligase complex.  With a predicted molecular weight of 70 kDa, human KEAP1 IS 74% identical to its mouse and rat orthologues.  KEAP1 contains an N-terminal BB Domain that mediates interaction with CUL3, a central BACK Domain that plays a role in protein-protein interactions, and six C-terminal Kelch Domains that function in substrate recognition.  KEAP1 is an important sensor of oxidative and electrophilic stress.  Normally the KEAP1/CUL3/RBX1 complex ubiquitinates and causes degradation of NRF2, a transcription factor regulating expression of many cytoprotective genes.  Oxidative stress results in the inability of KEAP1 to ubiquitinate NRF2, leading to the latter proteins accumulation and subsequent upregulation of genes involved in cellular oxidative stress responses.  

Related Research Areas

• Stem Cell Transcription Factor Regulators
• Transcriptional Co-Repressors