Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF
Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF Summary
Product Specifications
| Human Guanylyl Cyclase C/GUCY2C (Ser21-Gln430) Accession # P25092.2 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10575-GC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Human Guanylyl Cyclase C Fc Chimera Protein (Catalog # 10575-GC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-115 kDa and 200-230 kDa, respectively.
Reconstitution Calculator
Background: Guanylyl Cyclase C/GUCY2C
Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family expressed by intestinal epithelial cells from the duodenum to rectum. It was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (1). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (2-4). Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a kinase and GC catalytic domain. The ECD of human GUCY2C shares 70% and 72% amino acid sequence identity with the ECD of mouse and rat GUCY2C, respectively. Endogenous ligands of GUCY2C include guanylin and uroguanylin (5). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis. GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (5-7).
- Lucas K. et al. (2000) Pharmacol. Rev. 52:375.
- Potter, L.R. (2011) Cell Signal. 23:1921.
- Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
- Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
- Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
- Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
- Gong, R. et al. (2011) Science 333:1642.
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