Recombinant Human HA-Ubiquitin Protein, CF
Recombinant Human HA-Ubiquitin Protein, CF Summary
Product Specifications
Met1 - Gly76 with N-terminal HA (YPYDVPDYA) tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
U-110
Formulation | Supplied as a solution in HEPES. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Ubiquitin
Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).
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Citations for Recombinant Human HA-Ubiquitin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The MYC2-PUB22-JAZ4 module plays a crucial role in jasmonate signaling in tomato
Authors: Wu, S;Hu, C;Zhu, C;Fan, Y;Zhou, J;Xia, X;Shi, K;Zhou, Y;Foyer, CH;Yu, J;
Molecular plant
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
The FANCI/FANCD2 complex links DNA damage response to R-loop regulation through SRSF1-mediated mRNA export
Authors: Olazabal-Herrero, A;He, B;Kwon, Y;Gupta, AK;Dutta, A;Huang, Y;Boddu, P;Liang, Z;Liang, F;Teng, Y;Lan, L;Chen, X;Pei, H;Pillai, MM;Sung, P;Kupfer, GM;
Cell reports
Species: N/A
Sample Types: DNA, Recombinant Protein, RNA
Applications: Bioassay -
Structure of the DDB1-AMBRA1 E3 ligase receptor complex linked to cell cycle regulation
Authors: Liu, M;Wang, Y;Teng, F;Mai, X;Wang, X;Su, MY;Stjepanovic, G;
Nature communications
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Smurf1 polyubiquitinates on K285/K282 of the kinases Mst1/2 to attenuate their tumor-suppressor functions
Authors: Xu, Y;Qu, M;He, Y;He, Q;Shen, T;Luo, J;Tan, D;Bao, H;Xu, C;Ji, X;Hu, X;Barkat, MQ;Zeng, LH;Wu, X;
The Journal of biological chemistry
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation
Authors: Yamanaka, S;Furihata, H;Yanagihara, Y;Taya, A;Nagasaka, T;Usui, M;Nagaoka, K;Shoya, Y;Nishino, K;Yoshida, S;Kosako, H;Tanokura, M;Miyakawa, T;Imai, Y;Shibata, N;Sawasaki, T;
Nature communications
Applications: In vitro assay -
PRPF19 facilitates colorectal cancer liver metastasis through activation of the Src-YAP1 pathway via K63-linked ubiquitination of MYL9
Authors: R Zhou, J Chen, Y Xu, Y Ye, G Zhong, T Chen, L Qiu
Cell Death & Disease, 2023-04-08;14(4):258.
Species: Human
Sample Types:
Applications: Bioassay -
Reciprocal regulatory balance within the CLEC16A-RNF41 mitophagy complex depends on an intrinsically disordered protein region
Authors: M Gingerich, J Zhu, B Chai, M Vincent, N Xie, V Sidarala, N Kotov, D Sahu, D Klionsky, S Schnell, S Soleimanpo
The Journal of Biological Chemistry, 2023-02-22;0(0):103057.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia
Authors: H Zhuang, Y Ren, C Mao, Y Zhong, Z Zhang, B Cao, Y Zhang, J Huang, G Xu, Z Huang, Y Xu, X Mao
The Journal of Biological Chemistry, 2022-08-01;298(9):102314.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro
Authors: H Liu, S Liu, H Yu, X Huang, Y Wang, L Jiang, X Meng, G Liu, M Chen, Y Jing, F Yu, B Wang, J Li
Molecular plant, 2022-06-24;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
BMI-1 regulates DNA end resection and homologous recombination repair
Authors: A Fitieh, AJ Locke, F Mashayekhi, F Khaliqdina, AK Sharma, IH Ismail
Cell Reports, 2022-03-22;38(12):110536.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
The E3 ligase TRAF4 Promotes IGF Signaling by Mediating Atypical Ubiquitination of IRS-1
Authors: W Yu, R Singh, Z Wang, BW O'Malley, P Yi
Oncogene, 2021-05-13;0(0):100739.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
DAZAP2 acts as specifier of the p53 response to DNA damage
Authors: MC Liebl, J Moehlenbri, H Becker, G Raddatz, SK Abdeen, RI Aqeilan, F Lyko, TG Hofmann
Nucleic Acids Research, 2021-03-18;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Ubiquitination -
Mutual regulation between OGT and XIAP to control colon cancer cell growth and invasion
Authors: HG Seo, HB Kim, JY Yoon, TH Kweon, YS Park, J Kang, J Jung, S Son, EC Yi, TH Lee, WH Yang, JW Cho
Cell Death Dis, 2020-09-29;11(9):815.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7
Authors: M Morel, KN Shah, W Long
J. Biol. Chem., 2020-04-28;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Ubiquitination -
CAND1 regulates lunapark for the proper tubular network of the endoplasmic reticulum
Authors: H Kajiho, Y Yamamoto, T Sakisaka
Sci Rep, 2019-09-11;9(1):13152.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Impaired plasma membrane localization of ubiquitin ligase complex underlies 3-M syndrome development
Authors: P Wang, F Yan, Z Li, Y Yu, SE Parnell, Y Xiong
J. Clin. Invest., 2019-07-25;130(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response
Authors: F Liang, AS Miller, S Longerich, C Tang, D Maranon, EA Williamson, R Hromas, C Wiese, GM Kupfer, P Sung
Nat Commun, 2019-06-28;10(1):2849.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains
Authors: M Di Rienzo, M Antonioli, C Fusco, Y Liu, M Mari, I Orhon, G Refolo, F Germani, M Corazzari, A Romagnoli, F Ciccosanti, B Mandriani, MT Pellico, R De La Torr, H Ding, M Dentice, M Neri, A Ferlini, F Reggiori, M Kulesz-Mar, M Piacentini, G Merla, GM Fimia
Sci Adv, 2019-05-08;5(5):eaau8857.
Species: Human
Sample Types: Recombinant Protein
Applications: Ubiquitination -
Mechanism for recycling tRNAs on stalled ribosomes
Authors: MCJ Yip, AFA Keszei, Q Feng, V Chu, MJ McKenna, S Shao
Nat. Struct. Mol. Biol., 2019-04-22;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Ubiquitination -
The potato cyst nematode effector RHA1B is a ubiquitin ligase and uses two distinct mechanisms to suppress plant immune signaling
Authors: J Kud, W Wang, R Gross, Y Fan, L Huang, Y Yuan, A Gray, A Duarte, JC Kuhl, A Caplan, A Goverse, Y Liu, LM Dandurand, F Xiao
PLoS Pathog., 2019-04-12;15(4):e1007720.
Applications: Ubiquitination -
Binding of FANCI-FANCD2 Complex to RNA and R-Loops Stimulates Robust FANCD2 Monoubiquitination
Authors: Z Liang, F Liang, Y Teng, X Chen, J Liu, S Longerich, T Rao, AM Green, NB Collins, Y Xiong, L Lan, P Sung, GM Kupfer
Cell Rep, 2019-01-15;26(3):564-572.e5.
Applications: Bioassay -
RNF126 Quenches RNF168 Function in the DNA Damage Response
Authors: L Zhang, Z Wang, R Shi, X Zhu, J Zhou, B Peng, X Xu
Genomics Proteomics Bioinformatics, 2018-12-04;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N
Authors: N Gan, ES Nakayasu, PJ Hollenbeck, ZQ Luo
Nat Microbiol, 2018-11-12;4(1):134-143.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast
Authors: GS Hewawasam, K Dhatchinam, M Mattingly, C Seidel, JL Gerton
Nucleic Acids Res., 2018-05-18;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
PCGF5 is required for neural differentiation of embryonic stem cells
Authors: M Yao, X Zhou, J Zhou, S Gong, G Hu, J Li, K Huang, P Lai, G Shi, AP Hutchins, H Sun, H Wang, H Yao
Nat Commun, 2018-05-15;9(1):1463.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
A Regulatory Module Controlling Homeostasis of a Plant Immune Kinase
Authors: J Wang, LE Grubb, J Wang, X Liang, L Li, C Gao, M Ma, F Feng, M Li, L Li, X Zhang, F Yu, Q Xie, S Chen, C Zipfel, J Monaghan, JM Zhou
Mol. Cell, 2018-01-18;0(0):.
Applications: Bioassay -
Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183
Authors: Y Wu, XP Guo, S Kanemoto, Y Maeoka, A Saito, R Asada, K Matsuhisa, Y Ohtake, K Imaizumi, M Kaneko
PLoS ONE, 2018-01-04;13(1):e0190407.
Applications: Bioassay -
Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis
Authors: LT Gou, JY Kang, P Dai, X Wang, F Li, S Zhao, M Zhang, MM Hua, Y Lu, Y Zhu, Z Li, H Chen, LG Wu, D Li, XD Fu, J Li, HJ Shi, MF Liu
Cell, 2017-05-25;0(0):.
Applications: Bioassay -
CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag
Authors: H Takeda, W Zhou, K Kido, R Suno, T Iwasaki, T Kobayashi, T Sawasaki
PLoS ONE, 2017-05-25;12(5):e0178246.
Applications: Enzyme Assay -
Bam-dependent deubiquitinase complex can disrupt germ-line stem cell maintenance by targeting cyclin A
Authors: S Ji, C Li, L Hu, K Liu, J Mei, Y Luo, Y Tao, Z Xia, Q Sun, D Chen
Proc. Natl. Acad. Sci. U.S.A., 2017-05-08;0(0):.
Species: Drosophila
Sample Types: Whole Cells
Applications: Bioassay -
Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation
Authors: X Wei, X Wang, J Zhan, Y Chen, W Fang, L Zhang, H Zhang
J. Cell Biol., 2017-04-13;0(0):.
Applications: Bioassay -
Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors
Authors: J Zhu, KY Lee, KA Jewett, HY Man, HJ Chung, NP Tsai
PLoS Genet, 2017-02-17;13(2):e1006634.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Arabidopsis E3 ubiquitin ligase PLANT U-BOX13 (PUB13) regulates chitin receptor LYSIN MOTIF RECEPTOR KINASE5 (LYK5) protein abundance
Authors: D Liao, Y Cao, X Sun, C Espinoza, CT Nguyen, Y Liang, G Stacey
New Phytol, 2017-02-14;0(0):.
Applications: Enzyme Assay -
The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase Function
Authors: S He, Y Cao, P Xie, G Dong, L Zhang
Sci Rep, 2017-02-07;7(0):41364.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
S.�pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity
Authors: Z Lv, KA Rickman, L Yuan, K Williams, SP Selvam, AN Woosley, PH Howe, B Ogretmen, A Smogorzews, SK Olsen
Mol. Cell, 2017-02-02;65(4):699-714.e6.
Applications: Ubiquitination -
Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway
Authors: Sylvie van Twest
Mol. Cell, 2016-12-13;0(0):.
Applications: Bioassay -
SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer
Oncogene, 2016-11-21;0(0):.
Applications: Bioassay -
Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradation
Sci Rep, 2016-08-03;6(0):30955.
Species: Human
Sample Types: Protein
Applications: Enzyme Assay -
The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
Proc Natl Acad Sci USA, 2016-05-31;113(24):6725-30.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
O-GlcNAcylation Antagonizes Phosphorylation of Cdh1 (CDC20 homologue 1)
Authors: J Tian, Q Geng, Y Ding, J Liao, MQ Dong, X Xu, J Li
J Biol Chem, 2016-04-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Tumour suppressor TRIM33 targets nuclear beta-catenin degradation.
Authors: Xue J, Chen Y, Wu Y, Wang Z, Zhou A, Zhang S, Lin K, Aldape K, Majumder S, Lu Z, Huang S
Nat Commun, 2015-02-02;6(0):6156.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Requirement of Smurf-mediated endocytosis of Patched1 in sonic hedgehog signal reception.
Authors: Yue, Shen, Tang, Liu-Ya, Tang, Ying, Tang, Yi, Shen, Qiu-Hong, Ding, Jie, Chen, Yan, Zhang, Zengdi, Yu, Ting-Tin, Zhang, Ying E, Cheng, Steven Y
Elife, 2014-06-12;3(0):.
Applications: Bioassay -
A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
FASEB J, 2013-12-26;28(4):1938-46.
Species: Human
Sample Types: Recombinant Protein
Applications: Ubiquitination -
Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation.
Authors: Iguchi, Masahiro, Kujuro, Yuki, Okatsu, Kei, Koyano, Fumika, Kosako, Hidetaka, Kimura, Mayumi, Suzuki, Norihiro, Uchiyama, Shinichi, Tanaka, Keiji, Matsuda, Noriyuki
J Biol Chem, 2013-06-10;288(30):22019-32.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
C. elegans ring finger protein RNF-113 is involved in interstrand DNA crosslink repair and interacts with a RAD51C homolog.
Authors: Lee, Hyojin, Alpi, Arno F, Park, Mi So, Rose, Ann, Koo, Hyeon-So
PLoS ONE, 2013-03-28;8(3):e60071.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay -
ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.
Authors: Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou Y
Mol Cell, 2013-02-28;49(6):1108-20.
Applications: Bioassay -
Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair.
Authors: Lee, T-H, Park, J-M, Leem, S-H, Kang, T-H
Oncogene, 2012-11-26;33(1):19-25.
Applications: Bioassay -
A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity.
Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay L
J Biol Chem, 2012-11-05;287(52):43410-6.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system.
Authors: Basters A, Ketscher L, Deuerling E, Arkona C, Rademann J, Knobeloch K, Fritz G
BMC Biotechnol, 2012-08-23;12(0):56.
Applications: Bioassay -
TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members.
Authors: Bell JL, Malyukova A, Holien JK
PLoS ONE, 2012-05-21;7(5):e37470.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay -
Functional analysis of NopM, a novel E3 ubiquitin ligase (NEL) domain effector of Rhizobium sp. strain NGR234.
Authors: Xin DW, Liao S, Xie ZP
PLoS Pathog., 2012-05-17;8(5):e1002707.
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