Recombinant Human HABP1/C1QBP Protein, CF Summary
Product Specifications
His75-Gln282, with an N-term 2-Met tag and a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5155-HB
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile deionized water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: HABP1/C1QBP
Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is an ubiquitous acidic glycoprotein that functions both in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 73 amino acid (aa) N-terminal propeptide (3). The 32 kDa mature human HABP1, which contains a MAM33-like sequence, shares 90% aa sequence identity with mouse and rat HABP1. HABP1 assembles into a doughnut shaped trimer with the negatively charged residues asymmetrically distributed on one face and lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 3, 6 - 8). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (9 - 14). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (15).
- Thakur, S.C. et al. (2006) J. Androl. 27:604.
- Peerschke, E.I.B. and B. Ghebrehiwet (2007) Immunobiology 212:333.
- Grebrehiwet, B. et al. (1994) J. Exp. Med. 179:1809.
- Jiang, J. et al. (1999) Proc. Natl. Acad. Sci. 96:3572.
- Rozanov, D.V. et al. (2002) J. Biol. Chem. 277:9318.
- Deb, T.B. and K. Datta (1996) J. Biol. Chem. 271:2206.
- Lim, B.L. et al. (1996) J. Biol. Chem. 271:26739.
- Waggoner, S.N. et al. (2007) J. Leukoc. Biol. 82 epub.
- Innamorati, G. et al. (2006) Cell. Signal. 18:761.
- Robles-Flores, M. et al. (2002) J. Biol. Chem. 277:5247.
- Sunayama, J. et al. (1994) Cell Death Differ. 11:771.
- Schaerer, M.T. et al. (2001) J. Biol. Chem. 276:26597.
- Peterson-Mahrt, S.K. et al. (1999) EMBO J. 18:1014.
- Chattopadhyay, C. et al. (2004) Nucleic Acids Res. 32:3632.
- Majumdar, M. et al. (2002) Biochem. Biophys. Res. Commun. 291:829.
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