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Recombinant Human HGF (NS0-expressed) Protein

Catalog #: 294-HGN
15 Citations 2 Reviews Datasheet / COA / SDS
GMP Version Available: 294-GMP
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Carrier Free

Catalog # Availability Size / Price Qty
294-HGN-005/CF
294-HGN-025/CF
294-HGN-250/CF
294-HGN-100/CF

With Carrier

Catalog # Availability Size / Price Qty
294-HGN-005
294-HGN-025
294-HGN-01M
294-HGN-250
294-HGN-100
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Recombinant Human HGF (NS0-expressed) Protein Bioactivity
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Product Details
Citations (15)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human HGF (NS0-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is equal to or less than 4.00 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human HGF protein
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession #
P14210.2
N-terminal Sequence
Analysis
alpha chain: No results obtained; Gln32 predicted
beta chain: Val495
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
alpha chain: 53.7 kDa beta chain: 26 kDa
SDS-PAGE
71-85 kDa, non-reducing conditions
56-65 kDa and 30-40 kDa, reducing conditions

Product Datasheets

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294-HGN (with carrier)

Product Datasheet
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SDS

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294-HGN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

294-HGN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

294-HGN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 µg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human HGF (NS0-expressed) Protein Bioactivity View Larger

Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to or less than 4.00 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5) Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%-94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

References
  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and Birchmeier, W. (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and Fordham, W.D. (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. 13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. 14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. 15. Urbanek, K. et al. (2005) Circ. Res. 97:663.
Long Name
Hepatocyte Growth Factor
Entrez Gene IDs
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
Alternate Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

Citations for Recombinant Human HGF (NS0-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Betaglycan sustains HGF/Met signaling in lung cancer and endothelial cells promoting cell migration and tumor growth
    Authors: Cervantes-Villagrana, RD;Mendoza, V;Hinck, CS;de la Fuente-León, RL;Hinck, AP;Reyes-Cruz, G;Vázquez-Prado, J;López-Casillas, F;
    Heliyon
    Species: Mouse, Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. MET Oncogene Enhances Pro-Migratory Functions by Counteracting NMDAR2B Cleavage
    Authors: Gallo, S;Vitacolonna, A;Comoglio, PM;Crepaldi, T;
    Cells
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. MET Oncogene Controls Invasive Growth by Coupling with NMDA Receptor
    Authors: S Gallo, A Vitacolonn, P Comoglio, T Crepaldi
    Cancers, 2022-09-11;14(18):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Detection and quantification of human-specific mRNA from hepatocyte-like cells derived from dental pulp using real-time polymerase chain reaction
    Authors: N Shimamura, K Fujii, S Ohkoshi
    Journal of oral biosciences, 2021-07-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. cAMP-dependent activation of the Rac guanine exchange factor P-REX1 by type I protein kinase A (PKA) regulatory subunits
    Authors: SR Adame-Garc, RD Cervantes-, LB Orduña-Cas, JC Del Rio, JS Gutkind, G Reyes-Cruz, SS Taylor, J Vázquez-Pr
    J. Biol. Chem., 2018-12-10;0(0):.
    Species: Human, Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer
    Authors: A Jahangiri, A Nguyen, A Chandra, MK Sidorov, G Yagnik, J Rick, SW Han, W Chen, PM Flanigan, D Schneidman, S Mascharak, M De Lay, B Imber, CC Park, K Matsumoto, K Lu, G Bergers, A Sali, WA Weiss, MK Aghi
    Proc. Natl. Acad. Sci. U.S.A., 2017-09-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3
    Authors: H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng
    Sci Rep, 2017-03-02;7(0):43736.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Human Stem Cell-Derived Endothelial-Hepatic Platform for Efficacy Testing of Vascular-Protective Metabolites from Nutraceuticals
    Authors: BC Narmada, YT Goh, H Li, S Sinha, H Yu, C Cheung
    Stem Cells Transl Med, 2016-10-07;6(3):851-863.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Anti-c-Met monoclonal antibody ABT-700 breaks oncogene addiction in tumors with MET amplification.
    Authors: Wang J, Goetsch L, Tucker L, Zhang Q, Gonzalez A, Vaidya K, Oleksijew A, Boghaert E, Song M, Sokolova I, Pestova E, Anderson M, Pappano W, Ansell P, Bhathena A, Naumovski L, Corvaia N, Reilly E
    BMC Cancer, 2016-02-16;16(1):105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Deregulated hepsin protease activity confers oncogenicity by concomitantly augmenting HGF/MET signalling and disrupting epithelial cohesion.
    Authors: Tervonen T, Belitskin D, Pant S, Englund J, Marques E, Ala-Hongisto H, Nevalaita L, Sihto H, Heikkila P, Leidenius M, Hewitson K, Ramachandra M, Moilanen A, Joensuu H, Kovanen P, Poso A, Klefstrom J
    Oncogene, 2015-07-13;0(0):ePub.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Tivantinib (ARQ197) displays cytotoxic activity that is independent of its ability to bind MET.
    Authors: Basilico C, Pennacchietti S, Vigna E, Chiriaco C, Arena S, Bardelli A, Valdembri D, Serini G, Michieli P
    Clin Cancer Res, 2013-03-26;19(9):2381-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
    Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
    PLoS ONE, 2010-08-11;5(8):e12101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Applications: ELISA (Standard)
  14. Hepatocyte growth factor and its role in the pathogenesis of retinal detachment.
    Authors: Jin M, Chen Y, He S, Ryan SJ, Hinton DR
    Invest. Ophthalmol. Vis. Sci., 2004-01-01;45(1):323-9.
    Species: Rabbit
    Sample Types: In Vivo
    Applications: In Vivo
  15. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
    Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
    Cancer Res., 2003-02-15;63(4):887-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay

FAQs

  1. Does Recombinant Human HGF (NS0-expressed) Protein (Catalog # 294-HGN or 294-HGN/CF) contain any tags?

    • Recombinant Human HGF (NS0-expressed) Protein (Catalog # 294-HGN or 294-HGN/CF) does not contain any tags. Please refer to the Source section on the product-specific page for sequence information.

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Reviews for Recombinant Human HGF (NS0-expressed) Protein

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Recombinant Human HGF (NS0-expressed) Protein
By Anonymous on 12/23/2019
Application: In vitro bioactivity in cell culture

c-met reporter assay

In vitro bioactivity in cell culture Recombinant Human HGF (NS0-expressed) Protein 294-HGN
Recombinant Human HGF (NS0-expressed) Protein
By Anonymous on 07/19/2019
Application: In vitro bioactivity in cell culture