Recombinant Human His6-E6AP/UBE3A Protein, CF Summary
Product Specifications
Met1 - Leu875 with a C-terminal 6-His tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E3-230
| Formulation | Supplied as a solution in HEPES, NaCl and TCEP. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: E6AP/UBE3A
Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).
- Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
- Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
- Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
- Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
- Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
- Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.
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