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Recombinant Human His6-NEDD4 Protein, CF

Catalog #: E3-290
1 Citation Datasheet / COA / SDS
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E3-290-050
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-NEDD4 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human NEDD 4 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1)  enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.
Source
Trichoplusia ni, T. ni (baculovirus)-derived human NEDD4 protein
Leu570 - Asp1319 with a N-terminal Met and 6-His tag
Accession #
P46934.4
Predicted Molecular Mass
88 kDa

Product Datasheets

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E3-290

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-290

Formulation Supplied as a solution in HEPES, NaCl, DTT and EDTA.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEDD4

NEDD4 (Neural precursor cell expressed developmentally down-regulated protein 4) is a Ubiquitin E3 ligase and the prototypical member of an extended family of HECT domain ligases.  Human family members include NEDD4, NEDD4-2, ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDDL1 and NEDDL2.  In addition to the HECT domain, NEDD4 family members also contain a C2 domain and one or more WW domains.  With a predicted molecular weight of 150 kDa, NEDD4 is expressed in most human tissues where it localizes primarily to the perinuclear and other membrane regions.  Evidence suggests that NEDD4 down-regulates multiple membrane proteins (including FGFR1, IGFR1, and multiple EGFR family members) in a Ubiquitin-dependent internalization and lysosomal degradation process.  NEDD4 also plays a role in facilitating the sequestration of alpha-synuclein to cellular endosomes.  

References
  1. Harvey, K.F. and S. Kumar (1999) Trends Cell Biol. 9:166.
  2. Maspero, E., et al. (2013) Nat. Struct. Mol. Biol. 20:696.
  3. Scheffner, M. and S. Kumar (2014) Biochim. Biophys. Acta. 1843:61.
  4. Sugeno, N., et al. (2014) J. Biol. Chem. 289:18137.
  5. Yang, B. and S. Kumar (2010) Cell Death Differ. 17:68.
Long Name
Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4
Entrez Gene IDs
4734 (Human); 17999 (Mouse); 25489 (Rat)
Alternate Names
Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; EC 6.3.2; EC 6.3.2.-; KIAA0093NEDD4-1MGC176705; NEDD4; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated 4; receptor-potentiating factor 1; RPF1

Citation for Recombinant Human His6-NEDD4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Monitoring ?-synuclein ubiquitination dynamics reveals key endosomal effectors mediating its trafficking and degradation
    Authors: Zenko, D;Marsh, J;Castle, AR;Lewin, R;Fischer, R;Tofaris, GK;
    Science advances
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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