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Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF

Catalog #: E-554 Datasheet / COA / SDS

Discontinued Product

E-554 has been discontinued.
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Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-Otubain-2 Isoform 1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Otubain-2 Isoform 1 concentration of 0.1-2 μM.
Source
E. coli-derived human Otubain-2 protein
Contains a C-terminal 6-His tag
Accession #
Q96DC9-1
Predicted Molecular Mass
28 kDa

Product Datasheets

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E-554

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-554

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Otubain-2

Otubain-2 is a C65 type peptidase and a member of the ovarian tumor (OTU) protein super-family found in eukaryotes, viruses and pathogenic bacterium. Otubain-2 associates with the E3 ubiquitin ligases TRAF3 and TRAF6, and has been shown to negatively regulate virus-induced type I IFN induction and the antiviral responses mediated by these ligases. This recombinant protein contains a C-terminal 6-His tag.

References
  1. Li S., et al. (2010) J.Biol.Chem. 285: 4291-4297
  2. Nanao M.H., et al. (2004) EMBO Reports 5: 783-788
Long Name
OTU Domain, Ubiquitin Aldehyde Binding 2
Entrez Gene IDs
78990 (Human); 68149 (Mouse); 314405 (Rat)
Alternate Names
C14orf137; C14orf137chromosome 14 open reading frame 137; Deubiquitinating enzyme OTUB2; EC 3.4.19.12; FLJ21916; MGC3102; OTB2otubain-2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; OTU2; OTU2ubiquitin thioesterase OTUB2; OTUB2; Otubain2; Otubain-2; ubiquitin-specific protease otubain 2; Ubiquitin-specific-processing protease OTUB2

FAQs

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