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Recombinant Human His6-OTUD7B/Cezanne Protein, CF

Catalog #: E-562
1 Citation Datasheet / COA / SDS

Discontinued Product

E-562 has been discontinued.
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Recombinant Human His6-OTUD7B/Cezanne Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human OTUD7B/Cezanne is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human OTUD7B/Cezanne concentration of 0.1-1 μM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human OTUD7B/Cezanne protein
Contains a C-terminal 6-His tag
Accession #
Q6GQQ9
Predicted Molecular Mass
93 kDa

Product Datasheets

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E-562

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-562

Formulation

X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OTUD7B/Cezanne

OTU domain-containing protein 7B (OTUD7B), also known as cellular zinc finger anti-NF-kappaB (Cezanne), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 93 kDa (1). The human protein shares 90% amino acid sequence identity with its mouse ortholog. Cezanne has been reported to cleave K48-, and K63-linked poly-Ubiquitin chains, and also to display a preference for K11-linked chains (1-3). It has been shown to play a role in the negative regulation of NF-kappa B signaling, possibly by deubiquitination of RIPK1 and TRAF3 (2,4).

References
  1. Evans, P.C. et al. (2003) J. Biol. Chem. 278: 23180.
  2. Enesa, K. et al. (2008) J. Biol. Chem. 283: 7036.
  3. Bremm, A. et al. (2010) Nat. Struct. Mol. Biol. 17: 939.
  4. Hu, H. et al. (2013) Nature 494:371.
Long Name
OTU Domain-containing Protein 7B
Entrez Gene IDs
56957 (Human); 229603 (Mouse); 310677 (Rat)
Alternate Names
cellular zinc finger NF-kappaB Cezanne; Cellular zinc finger NF-kappa-B protein; Cezanne; CEZANNEA20 domain containing 1; EC 3.4.19.12; OTU domain containing 7B; OTU domain-containing protein 7B; OTUD7B; ZA20D1; Zinc finger A20 domain-containing protein 1; Zinc finger protein Cezanne

Citation for Recombinant Human His6-OTUD7B/Cezanne Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1
    Authors: LD Harris, J Le Pen, N Scholz, J Mieszczane, N Vaughan, S Davis, G Berridge, BM Kessler, M Bienz, JDF Licchesi
    The Journal of Biological Chemistry, 2021-01-08;296(0):100246.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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