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Recombinant Human His6-PA28 Activator gamma Subunit, CF

Catalog #: E-384 Datasheet / COA / SDS

Discontinued Product

E-384 has been discontinued.
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Recombinant Human His6-PA28 Activator gamma Subunit, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-PA28 gamma activates the trypsin-like (but not the chymotrypsin-like or caspase-like) activity of latent 20S Proteasome complexes. Reaction conditions will need to be optimized for each specific application.
Source
E. coli-derived human PA28 Activator gamma Subunit protein
Contains an N-terminal MSYY and 6-His tag
Accession #
P61289
Predicted Molecular Mass

31 kDa (monomer)

188 kDa (hexamer)

Product Datasheets

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E-384

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-384

Formulation

X mg/ml (X µM) in 50 mM HEPES pH 8.0, 500 mM NaCl, 0.1 mM EDTA, 1mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PA28 Activator gamma Subunit

The Proteasome Activator 28 (PA28) Regulatory Complex, also known as the 11S Regulatory (REG) Complex, is a ring-shaped, multimeric, ATP-independent regulatory complex that can bind to one or both ends of the 20S Proteasome or 20S Immunoproteasome (1,2). The PA28 Regulatory Complex stimulates the proteasome to hydrolyze small peptides, but not ubiquitinated proteins, for the production of peptides for MHC class I antigen presentation (3-7). Three PA28 Regulatory Complex subunits, alpha, beta, and gamma, have been identified and can oligomerize to form the PA28 Regulatory Complex (8-10). While the expression of the PA28 alpha and beta subunits are induced by IFN-gamma, the PA28 gamma subunit is not significantly affected (11,12).

The human PA28 Activator gamma Subunit, also known as 11S REG gamma and Ki antigen, is a 254 amino acid (aa) member of the PA28 family of regulatory proteins with a predicted molecular weight of 29 kDa. The mouse and rat orthologs share 100% aa sequence identity with human PA28 gamma. Two isoforms of PA28  gamma have been identified (11). Six PA28 gamma subunits combine to form a homo-hexameric ring-shaped PA28 Regulatory Complex (4). Unlike PA28 Regulatory Complexes composed of PA28 alpha subunits or PA28 alpha/beta subunits, regulatory complexes composed of PA28 gamma have narrow substrate specificity and have been shown to cleave only a few biologically relevant protein substrates including SRC-3, p21/CIP1/CDKN1A, and MafA (9,13-15). PA28 gamma is involved in cell cycle progression and in T cell-restricted MHC class I presentation of select antigens (16,17).

References
  1. Cascio, P. et al. (2002) EMBO J. 21:2636.
  2. Shibatani, T. et al. (2006) Mol. Biol. Cell 17:4962.
  3. Ma, C.P. et al. (1992) J. Biol. Chem. 267:10515.
  4. Wilk, S. et al. (2000) Arch. Biochem. Biophys. 383:265.
  5. Knowlton, J.R. et al. (1997) Nature 390:639.
  6. Groettrup, M. et al. (1996) Nature 381:166.
  7. Dick, T.P. et al. (1996) Cell 86:253.
  8. Kuehn, L. & B. Dahlmann (1997) Mol. Biol. Rep. 24:89.
  9. Li, J. & M. Rechsteiner (2001) Biochimie 83:373.
  10. Realini, C. et al. (1997) J. Biol. Chem. 272:25483.
  11. Jiang, H. & J.J. Monaco (1997) Immunogenetics 46:93.
  12. Ahn, J.Y. et al. (1995) FEBS Lett. 366:37.
  13. Li, X. et al. (2006) Cell 124:381.
  14. Kanai, K. et al. (2011) J. Mol. Endocrinol. 47:119.
  15. Chen, X. et al. (2007) Mol. Cell 26:843.
  16. Murata, S. et al. (1999) J. Biol. Chem. 274:38211.
  17. Barton, L.F. et al. (2004) J. Immunol. 172:3948.
Long Name
Proteasome (Prosome, Macropain) Activator Subunit 3
Entrez Gene IDs
10197 (Human)
Alternate Names
11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; Ki antigen; Ki; PA28 Activator gamma Subunit; PA28g; PA28gamma; PA28-gamma; PA28GKi nuclear autoantigen; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); Proteasome activator 28 subunit gamma; proteasome activator 28-gamma; proteasome activator complex subunit 3; PSME3; REG gamma; REG-GAMMA

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