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Recombinant Human His6-UBE2R1/CDC34 Protein, CF

Catalog #: E2-610
8 Citations Datasheet / COA / SDS

Discontinued Product

E2-610 has been discontinued.
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Citations (8)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-UBE2R1/CDC34 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-UbcH3/Cdc34 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH3/Cdc34 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH3 protein
Contains an N-terminal Met and 6-His tag
Accession #
P49427
Predicted Molecular Mass
28 kDa

Product Datasheets

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E2-610

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-610

Formulation X mg/ml (μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 1 mM TCEP, 10% Glycerol (v/v)
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2R1/CDC34

Ubiquitin-conjugating Enzyme H3 (UbcH3), also known as Cell Division Cycle 34 (Cdc34), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UbcH3 has a predicted molecular weight of 26 kDa. The human protein shares 98% amino acid (aa) sequence identity with the mouse and rat orthologs. In addition to an E2 catalytic core domain, UbcH3 has two Ubiquitin binding sites, UBS1, from aa 205-215, and UBS2, from aa 216-225, and a C-terminal acidic tail domain (2,3). UbcH3 is required for efficient cell cycle progression in human cells (4). UbcH3 interacts with the SCF(Fbw7) and SCF(Skp2) Ubiquitin ligases (E3s) to target the key cell cycle proteins Myc and p27/Kip1, respectively, for ubiquitination and degradation (4,5). Furthermore, CK2 has been shown to phosphorylate human UbcH3 on Ser203, Ser222, and Ser231 in cycling cells (6). The proliferation of cancer cell lines is inhibited following treatment with a human UbcH3 inhibitor, suggesting that UbcH3 also plays a significant role in cancer (7). This protein has an N-terminal His6-tag.

References
  1. Plon, S.E. et al. (1993) Proc. Natl. Acad. Sci. USA 90:10484.
  2. Choi, Y.S. et al. (2010) J. Biol. Chem. 285:17754.
  3. Block, K. et al. (2005) Cell Cycle 4:1421.
  4. Butz, N. et al. (2005) Exp. Cell Res. 303:482.
  5. Popov, N. et al. (2010) Nat. Cell Biol. 12:973.
  6. Sadowski, M. et al. (2007) Biochem. J. 405:569.
  7. Ceccarelli, D.F. et al. (2011) Cell 145:1075.
Entrez Gene IDs
997 (Human); 216150 (Mouse)
Alternate Names
Cdc34; cell division cycle 34 homolog (S. cerevisiae); cell division cycle 34; E2-CDC34; EC 6.3.2.19; UBC3; UBE2R1; UBE2R1UBCH3; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1

Citations for Recombinant Human His6-UBE2R1/CDC34 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The unique structure of the highly conserved PPLP region in HIV-1 Vif is critical for the formation of APOBEC3 recognition interfaces
    Authors: Iwatani, Y;Matsuoka, K;Ode, H;Kubota, M;Nakata, Y;Setoyama, Y;Kojima, K;Imahashi, M;Yokomaku, Y;
    mBio
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF
    Authors: Radko-Juettner, S;Yue, H;Myers, JA;Carter, RD;Robertson, AN;Mittal, P;Zhu, Z;Hansen, BS;Donovan, KA;Hunkeler, M;Rosikiewicz, W;Wu, Z;McReynolds, MG;Roy Burman, SS;Schmoker, AM;Mageed, N;Brown, SA;Mobley, RJ;Partridge, JF;Stewart, EA;Pruett-Miller, SM;Nabet, B;Peng, J;Gray, NS;Fischer, ES;Roberts, CWM;
    Nature
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Structure of CRL2Lrr1, the E3 ubiquitin ligase that promotes DNA replication termination in vertebrates
    Authors: H Zhou, MS Zaher, JC Walter, A Brown
    Nucleic Acids Research, 2021-12-16;49(22):13194-13206.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
    Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
    Cell Death & Disease, 2020-09-10;11(9):740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitylation
  5. Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors
    Authors: TR de Moura, S Mozaffari-, CZK Szabó, J Schmitzová, O Dybkov, C Cretu, M Kachala, D Svergun, H Urlaub, R Lührmann, V Pena
    Mol. Cell, 2018-03-15;69(6):979-992.e6.
    Species: Bacteria
    Sample Types: Cell Lysates
    Applications: Bioassay
  6. BAP1 regulates IP3R3-mediated Ca(2+) flux to mitochondria suppressing cell transformation
    Authors: A Bononi, C Giorgi, S Patergnani, D Larson, K Verbruggen, M Tanji, L Pellegrini, V Signorato, F Olivetto, S Pastorino, M Nasu, A Napolitano, G Gaudino, P Morris, G Sakamoto, LK Ferris, A Danese, A Raimondi, C Tacchetti, S Kuchay, HI Pass, EB Affar, H Yang, P Pinton, M Carbone
    Nature, 2017-06-14;546(7659):549-553.
    Applications: Bioassay
  7. SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S E2s to ubiquitylate ?-TrCP1 via K11-linkage for degradation
    Sci Rep, 2016-12-02;6(0):37441.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A subset of mixed lineage leukemia proteins has plant homeodomain (PHD)-mediated E3 ligase activity.
    Authors: Wang, Jingya, Muntean, Andrew G, Wu, Laura, Hess, Jay L
    J Biol Chem, 2012-11-05;287(52):43410-6.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination

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