Recombinant Human His6-USP2 Catalytic Domain Protein, CF
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Recombinant Human His6-USP2 Catalytic Domain Protein, CF Summary
Product Specifications
Asn259 - Met605 with a C-terminal 6-His tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-506
| Formulation | Supplied as a solution in HEPES, NaCl and TCEP. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: USP2
Ubiquitin Specific Peptidase 2 (USP2), also known as UBP41 and USP9, is a cytoplasmic protein that belongs to the peptidase C19 family of deubiquitinating enzymes (1). It is widely expressed and serves to deubiquitinate select target substrates. At least three isoforms have thus far been described: USP2a, also known as USP2-69, USP-41, and USP2-45 (2,3). All isoforms contain the same catalytic core, but show variations within their N- and C-terminal domains (3). USP2a is the canonical isoform. It is 605 amino acids (aa) in length and has a predicted molecular weight of 68.1 kDa. Human USP2a shares 90% aa sequence identity with the mouse and rat orthologs. USP-41 and USP-45 are 362 and 396 aa in length, respectively, and have predicted molecular weights of 41.6 and 45.2 kDa. USP-41 and USP2a have been shown to be critical regulators of apoptosis and cell proliferation. They deubiquitinate poly-ubiquitinated target proteins, such as FAS, MDM2, and Cyclin D1, to promote or block apoptosis, depending on the context (3-9). USP-45, whose expression appears to be regulated by circadian and nutritional cues, is thought to regulate hepatic gluconeogenesis and glucose metabolism (10,11). This recombinant human protein encompasses the catalytic domain of USP2.
- Baek, S.H. et al. (1997) J. Biol. Chem. 272:25560.
- Gousseva, N. & R.T. Baker (2003) Gene Expr. 11:163.
- Haimerl, F. et al. (2009) J. Biol. Chem. 284:495.
- Gewies, A. & S. Grimm (2003) Cancer Res. 63:682.
- Graner, E. et al. (2004) Cancer Cell 5:253.
- Stevenson, L.F. et al. (2007) EMBO J. 26:976.
- Shan, J. et al. (2009) Mol. Cell 36:469.
- Mahul-Mellier, A.L. et al. (2012) Cell Death Differ. 19:891.
- Mahul-Mellier, A.L. et al. (2012) Biochim. Biophys. Acta. 1823:1353.
- Molusky, M.M. et al. (2012) Diabetes 61:1025.
- Molusky, M.M. et al. (2012) PLoS One 7:e47970.
Citations for Recombinant Human His6-USP2 Catalytic Domain Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away
Authors: TET Mevissen, AV Prasad, JC Walter
Cell Reports, 2023-02-18;42(2):112125.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay -
Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes
Authors: S Yoon, K Bogdanov, D Wallach
Cell Death and Differentiation, 2022-01-09;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Usp9X Is Required for Lymphocyte Activation and Homeostasis through Its Control of ZAP70 Ubiquitination and PKC? Kinase Activity
Authors: E Naik, VM Dixit
J Immunol, 2016-03-02;196(8):3438-51.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay
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