Recombinant Human Hrk Western Blot Standard Protein

Recombinant Human Hrk Western Blot Standard Protein Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Hrk

HRK (harakiri) is a 10 kDa pro-apoptotic member of the Bcl-2 family of apoptosis regulatory proteins. Hrk lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 domain of Bik (Bcl-2-interacting Killer). Hrk physically interacts with Bcl-2 and Bcl-xL, but not with the pro-apoptotic Bax, BAK, or Bcl-xS. Hrk regulates apoptosis by interacting selectively with survival-promoting Bcl-2 and Bcl-xL to induce cell death.

Related Research Areas

• Bcl-2 Proteins and Regulators