Recombinant Human HRP-2 Isoform 1 Protein, CF Summary
Product Specifications
Pro2-Ser671, with a C-terminal 6-His tag
Analysis
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Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4956-HD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: HRP-2
Hepatoma-derived growth factor (HDGF)-related protein 2 (HRP-2) is a 74 kDa (predicted) member of the HDGF protein family (1 - 5). Similar to the electrophoretic mobility of HRP-3, HRP-2 runs as an anomalously large molecule in SDS-PAGE (4, 5). Members of this gene family have a highly conserved 98 amino acid (aa) sequence at the amino terminus (hath region, for homologous to amino terminus of HDGF) (1). Human HRP-2 is 671 aa in length and has no conserved sequence other than the hath region (1). There is no putative signal peptide sequence for secretion. Located within the hath region is a PWWP domain (aa 5 - 76), named after a conserved Pro-Trp-Trp-Pro motif. The protein also contains a serine-rich region (aa 142 - 269), an arginine-rich region (aa 318 - 391), an HIV-1 Integrase binding domain (IBD) (aa 470-552), a coiled-coil region (aa 521 - 581), and 19 phosphoserine sites that are apparently utilized. There are two isoforms for HRP-2, named 1 and 2. Isoform 2 is missing Ser640 which is found in isoform 1. Human HRP-2 shares 81% and 80% aa sequence identity with mouse and rat HRP-2, respectively. HRP-2 mRNA is highly expressed in testis and skeletal muscle, and is known to exist in neurons, astrocytes and oligodendroglia (1, 5). Like other proteins with a PWWP domain, HRP-2 is imported into the nucleus where it may function as a regulatory protein (1 - 2). The phosphoprotein may also play a role in viral replication, as it has been shown to bind to HIV-Integrase and efficiently reconstitute the in vitro activity of HIV-1 preintegration complexes (2 - 3).
- Izumoto, Y. et al. (1997) Biochem. Biophys. Res. Commun. 283:26.
- Cherepanov, P. et al. (2004) J. Biol. Chem. 279:48883
- Vandegraaff, N. et al. (2006) Virology 346:415.
- Dietz, F. et al. (2002) Biochem. J. 366:491.
- El-Tahir, H.M. et al. (2006) BMC Neurosci. Jan 23; 7:6.
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