Home / ICAM-5 / Recombinant Human ICAM-5 Fc Chimera Protein, CF

Recombinant Human ICAM-5 Fc Chimera Protein, CF

Catalog #: 1950-M5
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1950-M5-050

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human ICAM-5 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to Recombinant Human ICAM-5/Fc Chimera coated plates (25 µg/mL, 100 µL/well), >25% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ICAM-5 protein
Human ICAM-5
(Ala28-Glu570)
Accession # Q9UMF0		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
Q9UMF0
N-terminal Sequence
Analysis
Ala28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85.1 kDa (monomer)
SDS-PAGE
125-130 kDa, reducing conditions

Product Datasheets

You must select a language.

x

1950-M5

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1950-M5

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Background: ICAM-5

Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 consists of an 832 amino acid (aa) extracellular domain containing 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28 aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM-5 shares 38 - 55% aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity) and ICAM-3 (55% identity) (1). Human and mouse ICAM-5 share 85% aa identity.

The tissue distribution of ICAM-5 is unique among ICAMs, being expressed only in telencephalic regions of the central nervous system (2). Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) (3). Binding of ICAM-5 to LFA-1 is dependent on the first amino terminal Ig domain of ICAM-5 (4). ICAM-5 also displays homophilic binding, with the amino terminal Ig domain binding to Ig domains 4 - 5. Homophilic binding of ICAM-5 is dependent of ICAM-5 being in a monomeric form. The monomeric form of ICAM-5 is found during dendritogenesis in developing brain, whereas a high molecular weight complex is found in mature neurons (5).

References
  1. Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156.
  2. Yoshihara, Y. et al. (1994) Neuron 12:541.
  3. Tain, L. et al. (1997) J. Immunol. 158:928.
  4. Tain, L. et al. (2000) Eur. J. Immunol. 30:810.
  5. Tain, L. et al. (2000) J. Immunol. 150:243.
Long Name
Intercellular Adhesion Molecule 5
Entrez Gene IDs
7087 (Human); 15898 (Mouse)
Alternate Names
ICAM5; ICAM-5; intercellular adhesion molecule 5; intercellular adhesion molecule 5, telencephalin; Telencephalin; TLCNTelencephalin; TLNtelencephalin

Citations for Recombinant Human ICAM-5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Bypassing pan-enterovirus host factor PLA2G16
    Authors: J Baggen, Y Liu, H Lyoo, ALW van Vliet, M Wahedi, JW de Bruin, RW Roberts, P Overduin, A Meijer, MG Rossmann, HJ Thibaut, FJM van Kuppev
    Nat Commun, 2019-07-18;10(1):3171.
    Species: Virus
    Sample Types: Virus
    Applications: Bioassay
  2. ICAM-5/Telencephalin Is a Functional Entry Receptor for Enterovirus D68
    Authors: Wei Wei
    Cell Host Microbe, 2016-10-27;20(5):631-641.
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Human ICAM-5 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human ICAM-5 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human ICAM-5 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review