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Recombinant Human ICOS Fc Chimera Avi-tag Protein, CF

Catalog #: AVI10808 Datasheet / COA / SDS
Biotinylated
Catalog # Availability Size / Price Qty
AVI10808-050
Biotinylated Recombinant Human ICOS Fc Chimera Avi-tag Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ICOS Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H2 Fc Chimera Protein (Catalog # 165-B7) is coated at 100 ng/mL (100 μL/well), the concentration of Biotinylated Recombinant Human ICOS Fc Chimera Avi-tag (Catalog # AVI10808) that produces 50% of the optimal binding response is approximately 0.80-4.80 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human ICOS protein
Human ICOS
(Glu21-Gln134)
Accession # Q9Y6W8.1		IEGRMDHuman IgG1
(Pro100-Lys330)		Avi-tag
N-terminusC-terminus
Accession #
Q9Y6W8.1
N-terminal Sequence
Analysis
Glu21
Structure / Form
Disulfide-linked homodimer, biotinylated via Avi-tag
Predicted Molecular Mass
41 kDa
SDS-PAGE
49-62 kDa, under reducing conditions

Product Datasheets

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AVI10808

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10808

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human B7-H2 Fc Chimera Protein (165-B7) is coated at 100 ng/mL (100 μL/well), the concentration of Biotinylated Recombinant Human ICOS Fc Chimera Avi-tag (Catalog # AVI10808) that produces 50% of the optimal binding response is approximately 0.80 - 4.80 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human ICOS Fc Chimera Avi-tag (Catalog # AVI10808) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 49-62 kDa and 98-124 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation. Our Avi-tag Biotinylated ICOS Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein in unchanged so there is no interference in the protein's bioactivity.



References
  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Inducible Co-stimulator
Entrez Gene IDs
29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names
Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850

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