Recombinant Human IFN-gamma R1/CD119 Avi-tag His Protein, CF

Catalog #: AVI11030 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI11030-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-gamma R1/CD119 Avi-tag His Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinan human IFN-gamma (Catalog # 285-IF/CF) is immobilized at 2.00 µg/mL (100 µL/well), In the presence of Recombinant Human IFN-gamma R2, Biotinylated Recombinant Human IFN-gamma R1/CD119 Avi-tag His-tag binds with an ED₅₀ of 0.750-4.50 µg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IFN-gamma R1/CD119 protein

Human IFNGR1 (Glu18-Gly245) Accession # P15260.1	Avi-tag	6-His tag
N-terminus		C-terminus

Accession #

P15260.1

N-terminal Sequence
Analysis

Glu18 and Gly20

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

29 kDa

SDS-PAGE

42-55 kDa, under reducing conditions.

Product Datasheets

AVI11030

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11030

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant human IFN-gamma (285-IF/CF) is immobilized at 2.00 µg/mL (100 µL/well), In the presence of Recombinant Human IFN-gamma R2, Biotinylated Recombinant Human IFN-gamma R1/CD119 Avi-tag His-tag (Catalog # AVI11030) binds with an ED₅₀ of 0.750-4.50 µg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IFN‑ gamma R1/CD119 Avi-tag His-tag Protein (Catalog # AVI11030) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42‑55 kDa.

Reconstitution Calculator

Background: IFN-gamma R1/CD119

Interferon gamma receptor 1 (IFNGR1), along with IFNGR2, are type II cytokine receptors that combine to form a high affinity signaling complex with the type II interferon, IFNG. Mature human IFNGR1 consists of an extracellular domain (ECD) with 2 Ig-like domains, a transmembrane domain and an intracellular domain with both Jak1 and Stat1 binding motifs. The ECD of human IFNGR1 shares 50% amino acid sequence identity with mouse IFNGR1. The IFNG signaling complex is formed by 2 IFNGR1 subunits binding one IFNG dimer directly, and then 2 IFNGR2 molecules further stabilizing the receptor complex. Complex formation then triggers a signaling cascade that culminates in the transcription of the interferon stimulated genes (ISGs) and additional transcription factors. Ultimately, IFNGR1 mediated signaling regulates several biological processes including innate and acquired immune response, apoptosis and cell cycle progression. IFNGR1 is constitutively expressed in most cell types and deletions or mutations to IFNGR1 result in reduced resistance to bacterial, parasitic, and viral infection. Our Avi-tag Biotinylated IFNGR1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Bhat, M.Y. et al. (2018) J. Cell Commun Signal 12:745.
de Weerd, N.A. and Nguyen, T. (2012) Immunol Cell Biol. 90:483.
Mendoza, J.L. et al. (2019) Nature 567:56.
Blouin, C.M. and Lamaze, C. (2013) Front Immunol. 4:267.
Alspach, E. et al. (2019) Cold Spring Harb Perspect Biol. 11:a028480.

Long Name

Interferon gamma Receptor 1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Alternate Names

AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2