Recombinant Human IFN-gamma R1/CD119 His-tag Protein, CF

Catalog #: 11030-IR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11030-IR-100

Recombinant Human IFN-gamma R1/CD119 His-tag Protein SDS-PAGE.

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All IFN-gamma R1/CD119 Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-gamma R1/CD119 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human IFN-gamma (HEK293-expressed) (Catalog # 10067-IF) is immobilized at 3 µg/mL (100 µL/well), Recombinant Human IFN-gamma R1/CD119 His-tag (Catalog # 11030-IR) binds with an ED₅₀ of 0.0750-0.900 µg/mL.

Source

Human embryonic kidney cell, HEK293-derived human IFN-gamma R1/CD119 protein
Glu18-Gly245, with a C-terminal 6-His tag

Accession #

P15260.1

N-terminal Sequence
Analysis

Glu18

Predicted Molecular Mass

27 kDa

SDS-PAGE

36-46 kDa, under reducing conditions.

Product Datasheets

11030-IR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11030-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human IFN-gamma R1/CD119 His-tag Protein (Catalog # 11030-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-46 kDa.

Binding Activity

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When Recombinant Human IFN-gamma (HEK293-expressed) Protein (10067-IF) is immobilized at 3 µg/mL (100 µL/well), Recombinant Human IFN-gamma R1/CD119 His-tag Protein (Catalog # 11030-IR) binds with an ED₅₀ of 0.0750-0.900 µg/mL.

Reconstitution Calculator

Background: IFN-gamma R1/CD119

Interferon gamma receptor 1 (IFNGR1), along with IFNGR2, are type II cytokine receptors that combine to form a high affinity signaling complex with the type II interferon, IFNG. Mature human IFNGR1 consists of an extracellular domain (ECD) with 2 Ig-like domains, a transmembrane domain and an intracellular domain with both Jak1 and Stat1 binding motifs. The ECD of human IFNGR1 shares 50% amino acid sequence identity with mouse IFNGR1. The IFNG signaling complex is formed by 2 IFNGR1 subunits binding one IFNG dimer directly, and then 2 IFNGR2 molecules further stabilizing the receptor complex. Complex formation then triggers a signaling cascade that culminates in the transcription of the interferon stimulated genes (ISGs) and additional transcription factors. Ultimately, IFNGR1 mediated signaling regulates several biological processes including innate and acquired immune response, apoptosis and cell cycle progression. IFNGR1 is constitutively expressed in most cell types and deletions or mutations to IFNGR1 result in reduced resistance to bacterial, parasitic, and viral infection.

References

Bhat, M.Y. et al. (2018) J. Cell Commun Signal 12:745.
de Weerd, N.A. and Nguyen, T. (2012) Immunol Cell Biol. 90:483.
Mendoza, J.L. et al. (2019) Nature 567:56.
Blouin, C.M. and Lamaze, C. (2013) Front Immunol. 4:267.
Alspach, E. et al. (2019) Cold Spring Harb Perspect Biol. 11:a028480.

Long Name

Interferon gamma Receptor 1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Alternate Names

AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2