Recombinant Human IL-1 RAcP/IL-1 R3 Fc Avi-tag Protein, CF

Catalog #: AVI11139 Datasheet / COA / SDS

Biotinylated Fc Chimera

Catalog #	Availability	Size / Price	Qty
AVI11139-050

Biotinylated Recombinant Human IL‑1 RAcP/IL‑1 R3 Fc Chimera Avi-tag Protein Binding Activity.

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All IL-1 RAcP/IL-1 R3 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-1 RAcP/IL-1 R3 Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human IL-1 RII Fc Chimera (Catalog # 663-2R) is immobilized at 0.5 µg/mL (100 µL/well), in the presence of Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB), it binds to Biotinylated Recombinant Human IL-1 RAcP/IL-1 R3 Fc Chimera Avi-tag (Catalog # AVI11139) with an ED₅₀ of 4.00-36.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-1 RAcP/IL-1 R3 protein

Human IL-1RAP (Ser21-Glu359) Accession # Q9NPH3.2	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

Q9NPH3.2

N-terminal Sequence
Analysis

Ser21

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

68 kDa

SDS-PAGE

83 - 93 kDa, under reducing conditions.

Product Datasheets

AVI11139

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11139

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-1 RII Fc Chimera (663-2R) is immobilized at 0.5 µg/mL (100 µL/well), in the presence of Recombinant Human IL-1 beta /IL-1F2 (201-LB), it binds to Biotinylated Recombinant Human IL-1 RAcP/IL-1 R3 Fc Chimera Avi-tag Protein (Catalog # AVI11139) with an ED₅₀ of 4.00-36.0 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL‑1 RAcP/IL‑1 R3 Fc Chimera Avi-tag Protein (Catalog # AVI11139) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-93 kDa and 170-190 kDa, respectively.

Reconstitution Calculator

Background: IL-1 RAcP/IL-1 R3

IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins (1). It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36 (2-4). It is a subunit of the functional signaling complex with IL-1 RI and associates with IL-1 RII in a non-signaling receptor complex (2, 5). In addition, it interacts with ST2/IL-1 R4 on mast cells and Th2 cells to create a functional IL-33 receptor complex (3). IL-1 RAcP also functions as a co-receptor for IL-36 alpha /IL-1F6, IL-36 beta /IL-1F8, and IL-36 gamma /IL-1F9 (4). Mature human IL-1 RAcP consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (2). Within the ECD, human IL-1 RAcP shares 86% aa sequence identity with mouse and rat IL-1 RAcP. Alternative splicing generates two secreted decoy receptor isoforms and an isoform with a substituted cytoplasmic domain (6-8). When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (Our Avi-tag Biotinylated human IL-1 RAcP Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Boraschi, D. and A. Tagliabue (2013) Semin. Immunol. 25:394.
Greenfeder, S.A. et al. (1995) J. Biol. Chem. 270:13757.
Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Lang, D. et al. (1998) J. Immunol. 161:6871.
Lu, H.-L. et al. (2008) Mol. Immunol. 45:1374.
Jensen, L.E. et al. (2000) J. Immunol. 164:5277.
Jensen, L.E. and A.S. Whitehead (2003) Cell. Signal. 15:793.
Smith, D.E. et al. (2003) Immunity 18:87.
Yoshida, T. et al. (2012) J. Neurosci. 32:2588.

Long Name

Interleukin 1 Receptor Accessory Protein

Entrez Gene IDs

3556 (Human); 16180 (Mouse)

Alternate Names

C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3; FLJ37788; IL-1 R3; IL-1 RAcP; IL-1 receptor accessory protein; IL-1R3; IL-1R-3; IL1RAcP; IL-1RAcP; IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein beta; interleukin-1 receptor accessory protein