Recombinant Human IL-1 RAcP/IL-1 R3 Protein, CF

Catalog #: 9176-CP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9176-CP-100

Recombinant Human IL-1 RAcP/IL-1 R3 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-1 RAcP/IL-1 R3 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-1 alpha -induced IL-8 secretion in HepG2 human hepatocellular carcinoma cells. The ED₅₀ for this effect is 0.8‑4 µg/mL in the presence of 1 μg/mL of recombinant human (rh) IL-1 R2 Fc Chimera and 50 pg/mL rhIL-1 alpha.

Source

Human embryonic kidney cell, HEK293-derived human IL-1 RAcP/IL-1 R3 protein
Ser21-Glu359, with a C-terminal 6-His tag

Accession #

Q9NPH3

N-terminal Sequence
Analysis

Ser21

Predicted Molecular Mass

40 kDa

SDS-PAGE

55-65 kDa, reducing conditions

Product Datasheets

9176-CP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9176-CP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human IL-1 RAcP/IL-1 R3 (Catalog # 9176-CP) inhibits IL-1 alpha -induced IL-8 secretion in HepG2 human hepatocellular carcinoma cells. The ED₅₀ for this effect is 0.8-4 μg/mL.

Reconstitution Calculator

Background: IL-1 RAcP/IL-1 R3

IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins (1). It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36 (2-4). It is a subunit of the functional signaling complex with IL-1 RI and associates with IL-1 RII in a non-signaling receptor complex (2, 5). In addition, it interacts with ST2/IL-1 R4 on mast cells and Th2 cells to create a functional IL-33 receptor complex (3). IL-1 RAcP also functions as a co-receptor for IL-36 alpha /IL-1F6, IL-36 beta /IL-1F8, and IL-36 gamma /IL-1F9 (4). Mature human IL-1 RAcP consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (2). Within the ECD, human IL-1 RAcP shares 86% aa sequence identity with mouse and rat IL-1 RAcP. Alternative splicing generates two secreted decoy receptor isoforms and an isoform with a substituted cytoplasmic domain (6-8). When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (9). Neuronal IL-1 RAcP interacts trans-synaptically with PTP sigma and can induce excitatory pre- and post-synaptic development (10).

References

Boraschi, D. and A. Tagliabue (2013) Semin. Immunol. 25:394.
Greenfeder, S.A. et al. (1995) J. Biol. Chem. 270:13757.
Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Lang, D. et al. (1998) J. Immunol. 161:6871.
Lu, H.-L. et al. (2008) Mol. Immunol. 45:1374.
Jensen, L.E. et al. (2000) J. Immunol. 164:5277.
Jensen, L.E. and A.S. Whitehead (2003) Cell. Signal. 15:793.
Smith, D.E. et al. (2003) Immunity 18:87.
Yoshida, T. et al. (2012) J. Neurosci. 32:2588.

Long Name

Interleukin 1 Receptor Accessory Protein

Entrez Gene IDs

3556 (Human); 16180 (Mouse)

Alternate Names

C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3; FLJ37788; IL-1 R3; IL-1 RAcP; IL-1 receptor accessory protein; IL-1R3; IL-1R-3; IL1RAcP; IL-1RAcP; IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein beta; interleukin-1 receptor accessory protein