Recombinant Human IL-1 RI Avi-tag His-tag Protein, CF

Catalog #: AVI11319 Datasheet / COA / SDS

Biotinylated

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AVI11319-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-1 RI Avi-tag His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-1 RAcP/IL-1 R3 (Catalog # 9176-CP) is immobilized at 2 µg/mL (100 µL/well) in the presence of Recombinant Human IL-1 alpha /IL-1F1 (Catalog # 200-LA), it binds to Biotinylated Recombinant Human IL-1 RI Avi-tag His-tag (Catalog # AVI11319) with an ED₅₀ of 0.125-1.00 µg/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-1 RI protein

Human IL-1 RI (Asp21-Lys336) Accession # P14778.1	Avi-tag	6-His tag
N-terminus		C-terminus

Accession #

P14778.1

N-terminal Sequence
Analysis

Asp21

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

40 kDa

SDS-PAGE

55-66 kDa, under reducing conditions.

Product Datasheets

AVI11319

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11319

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-1 RAcP/IL-1 R3 (9176-CP) is immobilized at 2 µg/mL (100 µL/well) in the presence of Recombinant Human IL-1 alpha /IL-1F1 (200-LA), it binds to Biotinylated Recombinant Human IL-1 RI Avi-tag His-tag Protein (Catalog # AVI11319) with an ED₅₀ of 0.125-1.00 µg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL‑1 RI Avi-tag His-tag Protein (Catalog # AVI11319) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-66 kDa.

Reconstitution Calculator

Background: IL-1 RI

The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily (1 - 3). IL-1 RI is an 80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein (1, 2). This complex recruits the adaptor protein MyD88, to initiate signaling in the NF kappa B pathway (4, 5). Human IL-1 RI cDNA encodes a 569 amino acid (aa) protein that contains a 17 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 20 aa TM domain and a 213 aa cytoplasmic region with a TIR domain. Within the ECD domain, human IL-1 RI shares 63% and 64% aa identity with mouse and rat IL-1 RI, respectively. The role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and inhibitory IL-1 R isoforms and decoys (1 - 5). IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever (1, 2, 5, 6). Our Avi-tag Biotinylated human IL-1 R1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Boraschi, D. & A. Tagliabue (2006) Vitam. Horm. 74:229.
Dinarello, C.A. (2002) Clin. Exp. Rheumatol. 20:S1.
Hart, R.P. et al. (1993) J. Neuroimmunol. 44:49.
Brikos, C. et al. (2007) Mol. Cell. Proteomics 6:1551.
Gasse, P. et al. (2007) J. Clin. Invest. 117:3786.
Ching, S. et al. (2007) J. Neurosci. 27:10476.

Long Name

Interleukin 1 Receptor I

Entrez Gene IDs

3554 (Human); 16177 (Mouse)

Alternate Names

CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80