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Recombinant Human IL-13 R alpha 1 His-tag Protein, CF

Catalog #: 11424-IR Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11424-IR-100
Recombinant Human IL‑13 R alpha 1 His-tag Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-13 R alpha 1 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Human IL-13 R alpha 1 His-tag (Catalog # 11424-IR) binds Recombinant Human IL-13 (Catalog # 213-ILB/CF) with an ED50 of 37.5‑450 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-13 R alpha 1 protein
Ala27-Thr343, with a C-terminal 6-His tag
Accession #
P78552.1
N-terminal Sequence
Analysis
Ala27
Predicted Molecular Mass
37 kDa
SDS-PAGE
58-64 kDa, under reducing conditions.

Product Datasheets

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11424-IR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11424-IR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Recombinant Human IL‑13 R alpha 1 His-tag Protein (Catalog # 11424-IR) binds Recombinant Human IL-13 (213-ILB/CF) with an ED50 of 37.5-450 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human IL‑13 R alpha 1 His-tag Protein (Catalog # 11424-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-64 kDa, under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-13 R alpha 1

IL-13 RA1, also known as IL-13R and IL-13 RA, is a type I transmembrane protein. Its cDNA encodes a 427 aa precursor protein, with 322 aa extracellular domain, 24 aa transmembrane domain and 60 aa intracellular domain. Within the extracellular domain, human IL-13 RA1 shares 75% and 74% homology with mouse and rat IL-13 RA1, respectively. IL-13 RA1 expresses ubiquitously in all tissues with the highest level in heart, liver, skeletal muscle and ovary (1). As a receptor, IL-13 RA1 can function alone or as a heterodimer with IL-4R. Although both IL-4 and IL-13 signal through IL-4R/IL-13 RA1 heterodimer, there are distinct differences. IL-4 binds IL-4R with high affinity then binds IL-13 RA1 with low affinity. In contrast, IL-13 binds IL-13 RA1 with decent affinity, then binds IL-4R with high affinity (2). In addition, the N-terminal Fibronectin type III domain (D1) of IL-13 RA1 is only required for the binding of IL-13 not IL-4 (3,4). After binding to IL-4 or IL-13, the Tyr residues in the cytoplasmic domain of IL-13 RA get phosphorylated and then activate signaling proteins including Jak1, Tyk1, Tyk2, IRS-1, and STAT6 (5, 6). Alternative splicing generates soluble iL-13 RA1 missing the transmembrane domain (7). It not only functions as a decoy receptor for IL-13, but also is able to reduce fasting blood glucose, mediated by IL-4 (8). Higher expression of IL-13 RA1 are found in several cancers, often associated with poor prognosis in patients (9-11). 

References
  1. Aman M.J. et al. (1996) J. Biol. Chem. 271:29265.
  2. LaPorte S.L. et al. (2008) Cell 132:259.
  3. Arima K. et al. (2005) J. Biol. Chem. 280:24915.
  4. Ito T, et al. (2009) J. Biol. Chem. 284:24289.
  5. Umeshita-Suyama R. et al. (2000) Int. Immunol. 12:1499.
  6. Roy B, et al. (2002) J. Leukoc. Biol. 72:580.
  7. Osawa M, et al. (2000) Immunogenetics 51:974.
  8. Rachmin I, et al. (2017) Am. J. Physiol. Endocrinol. Metab. 313:E663.
  9. Park M.H. et al. (2017) Ann, Surg. Oncol. 24:3780.
  10. Cao H, et al. (2016) Oncotarget 7:61183.
  11. Suzuki A, et al. (2015) Cytokine 75:79.
Long Name
Interleukin 13 Receptor alpha 1
Entrez Gene IDs
3597 (Human); 16164 (Mouse)
Alternate Names
bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL-13 R alpha 1; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL13R alpha 1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13Ra1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4

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