Recombinant Human IL-17B (CHO) Protein Summary
Product Specifications
Gln21-Phe180
Analysis
Gln21 predicted, sequencing might be blocked
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8129-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate and NaCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8129-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Human IL-17B induces IL-8 secretion by HepG2 human hepatocellular carcinoma cells.
Reconstitution Calculator
Background: IL-17B
Interleukin-17B (IL-17B), is an approximately 20 kDa glycosylated cytokine that plays a role in inflammation and bone growth. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17B shares 90% and 91% amino acid sequence identity with mouse and rat IL-17B, respectively (3, 4). IL-17B binds to IL-17 RB and induces the production of inflammatory cytokines and the infiltration of inflammatory immune cells (3, 4). IL-17B is expressed in the central nervous system, testis, ovary, stomach, pancreas, and small intestine (3-5). In the skeletal system, IL-17B is expressed in bone collars surrounding primary ossification centers as well as in epiphyseal plate chondrocytes during development and after bone fracture (6, 7). IL-17B is up-regulated in arthritic cartilage where it exacerbates the severity of disease (8). It is also up-regulated in aggressive breast cancer cell lines, and its exaggerated signaling through IL-17 RB promotes tumorigenicity (9). It inhibits the adhesion of vascular endothelial cells to Matrigel and enhances their migration (10).
- Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
- Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
- Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
- Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
- Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.
- Kokubu, T. et al. (2008) J. Histochem. Cytochem. 56:89.
- You, Z. et al. (2005) Biochem. Biophys. Res. Commun. 326:624.
- Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
- Huang, C.-K. et al. (2013) Oncogene July 15 Epub.
- Sanders, A.J. et al. (2010) J. Oncol. 2010:817375.
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